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- W2023773244 abstract "Expression of the deo operon of Escherichia coli K12 has been studied in vitro using the Zubay (1973) system for DNA primed transcription and translation. The kinetics for messenger RNA synthesis of all four structural deo genes have been followed by use of rifampicin and streptolydigin. The results show that all three deo promoters initiate mRNA synthesis in vitro; expression of the internal deo promoter was demonstrated using a phage lambda DNA template constructed so as to be devoid of the other two promoters. The relative amounts of mRNA made from the three deo promoters in vitro correspond to previous findings in vivo. At one of the deo promoters initiation of transcription starts immediately whereas a lag in initiation is seen for the two other deo promoters and also for the lac promoter. This demonstrates that the times required for RNA polymerase to reach a constant rate of initiation from the three deo promoters in vitro are different. The time lag in initiation of transcription, characteristic for a given deo promoter, is found not to be inversely proportional to the promoter strength." @default.
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- W2023773244 date "1979-09-01" @default.
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- W2023773244 title "Evidence for the existence of three promoters for the deo operon of Escherichia coli K12 in vitro" @default.
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- W2023773244 doi "https://doi.org/10.1016/0022-2836(79)90248-1" @default.
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