Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023774467> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2023774467 endingPage "15929" @default.
- W2023774467 startingPage "15924" @default.
- W2023774467 abstract "A symmetrical cyanine dye was previously shown to bind as a cofacial dimer to alternating A-T sequences of duplex DNA. Indirect evidence suggested that dimerization of the dye occurred in the minor groove. 1H NMR experiments reported here verify this model based on broadening and shifting of signals due to protons on carbon 2 of adenine and imino protons at the central five A-T pairs of the 11 base pair duplex: 5'-GCGTATATGCG-3'/3'-CGCATATACGC-5'. This binding mode is similar to that of distamycin A, even though the dye lacks the hydrogen-bonding groups used by distamycin for sequence-specific recognition. Surprisingly, the third base pair (G-C) was also implicated in the binding site. UV-vis experiments were used to compare the extent of dimerization of the dye for 11 different sequence variants. These experiments verified the importance of a G-C pair at the third position: replacing this pair with A-T suppressed dimerization. These results indicate that the dye binding site spans six base pairs: 5'-GTATAT-3'. The initial G-C pair seems to be important for widening the minor groove rather than for making important contacts with the dye molecules since inverting its orientation to C-G or replacing it with I-C still led to favorable dimerization of the dye." @default.
- W2023774467 created "2016-06-24" @default.
- W2023774467 creator A5022491754 @default.
- W2023774467 creator A5038997100 @default.
- W2023774467 creator A5084710230 @default.
- W2023774467 date "2005-11-08" @default.
- W2023774467 modified "2023-09-25" @default.
- W2023774467 title "<sup>1</sup>H NMR and Optical Spectroscopic Investigation of the Sequence-Dependent Dimerization of a Symmetrical Cyanine Dye in the DNA Minor Groove" @default.
- W2023774467 cites W1972459558 @default.
- W2023774467 cites W2003464598 @default.
- W2023774467 cites W2027583545 @default.
- W2023774467 cites W2039993377 @default.
- W2023774467 cites W2151226445 @default.
- W2023774467 cites W2313098582 @default.
- W2023774467 cites W273801837 @default.
- W2023774467 doi "https://doi.org/10.1021/bi051298e" @default.
- W2023774467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16313195" @default.
- W2023774467 hasPublicationYear "2005" @default.
- W2023774467 type Work @default.
- W2023774467 sameAs 2023774467 @default.
- W2023774467 citedByCount "24" @default.
- W2023774467 countsByYear W20237744672012 @default.
- W2023774467 countsByYear W20237744672013 @default.
- W2023774467 countsByYear W20237744672014 @default.
- W2023774467 countsByYear W20237744672015 @default.
- W2023774467 countsByYear W20237744672018 @default.
- W2023774467 countsByYear W20237744672020 @default.
- W2023774467 countsByYear W20237744672022 @default.
- W2023774467 countsByYear W20237744672023 @default.
- W2023774467 crossrefType "journal-article" @default.
- W2023774467 hasAuthorship W2023774467A5022491754 @default.
- W2023774467 hasAuthorship W2023774467A5038997100 @default.
- W2023774467 hasAuthorship W2023774467A5084710230 @default.
- W2023774467 hasConcept C103319777 @default.
- W2023774467 hasConcept C112887158 @default.
- W2023774467 hasConcept C121332964 @default.
- W2023774467 hasConcept C178790620 @default.
- W2023774467 hasConcept C185581394 @default.
- W2023774467 hasConcept C185592680 @default.
- W2023774467 hasConcept C2777324032 @default.
- W2023774467 hasConcept C2778112365 @default.
- W2023774467 hasConcept C2779546866 @default.
- W2023774467 hasConcept C3019303373 @default.
- W2023774467 hasConcept C32909587 @default.
- W2023774467 hasConcept C552990157 @default.
- W2023774467 hasConcept C55493867 @default.
- W2023774467 hasConcept C62520636 @default.
- W2023774467 hasConcept C71240020 @default.
- W2023774467 hasConcept C8010536 @default.
- W2023774467 hasConcept C91881484 @default.
- W2023774467 hasConcept C99611785 @default.
- W2023774467 hasConceptScore W2023774467C103319777 @default.
- W2023774467 hasConceptScore W2023774467C112887158 @default.
- W2023774467 hasConceptScore W2023774467C121332964 @default.
- W2023774467 hasConceptScore W2023774467C178790620 @default.
- W2023774467 hasConceptScore W2023774467C185581394 @default.
- W2023774467 hasConceptScore W2023774467C185592680 @default.
- W2023774467 hasConceptScore W2023774467C2777324032 @default.
- W2023774467 hasConceptScore W2023774467C2778112365 @default.
- W2023774467 hasConceptScore W2023774467C2779546866 @default.
- W2023774467 hasConceptScore W2023774467C3019303373 @default.
- W2023774467 hasConceptScore W2023774467C32909587 @default.
- W2023774467 hasConceptScore W2023774467C552990157 @default.
- W2023774467 hasConceptScore W2023774467C55493867 @default.
- W2023774467 hasConceptScore W2023774467C62520636 @default.
- W2023774467 hasConceptScore W2023774467C71240020 @default.
- W2023774467 hasConceptScore W2023774467C8010536 @default.
- W2023774467 hasConceptScore W2023774467C91881484 @default.
- W2023774467 hasConceptScore W2023774467C99611785 @default.
- W2023774467 hasIssue "48" @default.
- W2023774467 hasLocation W20237744671 @default.
- W2023774467 hasLocation W20237744672 @default.
- W2023774467 hasOpenAccess W2023774467 @default.
- W2023774467 hasPrimaryLocation W20237744671 @default.
- W2023774467 hasRelatedWork W187039139 @default.
- W2023774467 hasRelatedWork W2013659604 @default.
- W2023774467 hasRelatedWork W2016943609 @default.
- W2023774467 hasRelatedWork W2023774467 @default.
- W2023774467 hasRelatedWork W2032188169 @default.
- W2023774467 hasRelatedWork W2033018937 @default.
- W2023774467 hasRelatedWork W2045132616 @default.
- W2023774467 hasRelatedWork W2079346183 @default.
- W2023774467 hasRelatedWork W2509864854 @default.
- W2023774467 hasRelatedWork W3094440071 @default.
- W2023774467 hasVolume "44" @default.
- W2023774467 isParatext "false" @default.
- W2023774467 isRetracted "false" @default.
- W2023774467 magId "2023774467" @default.
- W2023774467 workType "article" @default.