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- W2023783979 abstract "We have utilized a replica transfer technique to develop a novel screening assay for the identification of transfectants expressing β2-adrenergic receptors (β2-AR). The hamster β2-AR gene flanked by either its natural promoter or the zinc-inducible mouse metailothionein (MMT) promoter was cotransfected with plasmids conferring neomycin resistance (pRSVneo) into β2-AR-deficient mouse L cells. Transfectant colonies were grown on polyester nylon filters and screened by filter binding with [125I]iodohydroxybenzylpindolol to identify colonies expressing β2-AR. Individual colonies were isolated and examined to determine β2-AR gene dosage, mRNA expression, and receptor densities and affinities. Analysis of cell lines expressing β2-AR indicates that this method can identify transfectants containing only a single β2-AR gene copy and expressing as few as 4,000 β2-receptors per cell. This method may be useful as a tool for the molecular cloning of neuro-transmitter receptor genes and for the measurement of transfection efficiencies and expression of receptor genes in cells." @default.
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- W2023783979 date "1989-07-01" @default.
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- W2023783979 title "Replica Filter Screening Technique to Detect Transfected Cells Expressing β<sub>2</sub>-Adrenergic Receptor" @default.
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- W2023783979 doi "https://doi.org/10.1089/dna.1.1989.8.447" @default.
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