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- W2023786329 abstract "We explain the use of a method for labelling transfer RNAs with Co+++, a covalent analog of magnesium. We show that this labelling is stable after hydrolysis of yeast tRNAPhe by T1 ribonuclease, and suggest that such a method could be developed to resolve tRNA tertiary structure. Nous exposons l'utilisation d'une méthode de marquage des ARN de transfert par l'ion cobaltique, analogue covalent du magnésium. Nous montrons que le marquage est stable après hydrolyse de l'ARNtPhe de la levure par la ribonucléase T1 et nous suggérons qu'une telle méthode de marquage pourrait être développée pour résoudre la structure tertiaire des ARN de transfert. Wir beschreiben die Anwendung einer Markierungsmethode der Transfer-Ribonukleinsäuren mit dem Kobaltion, einem kovalenten Analogon des Magnesiums. Wir zeigen, daß die Markierung nach der Hydrolyse der tRNSPhe der Hefe durch die Ribonuklease T1 stabil ist, und wir zeigen, dass eine derartige Methode weiterentwickelt werden könnte, um die tertiäre Struktur der tRNS zu deuten." @default.
- W2023786329 created "2016-06-24" @default.
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- W2023786329 date "1972-01-01" @default.
- W2023786329 modified "2023-09-24" @default.
- W2023786329 title "A new method for specific labelling of tRNA : Preliminary results on yeast tRNAPhe" @default.
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- W2023786329 doi "https://doi.org/10.1016/s0300-9084(72)80212-8" @default.
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