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• W2023790596 endingPage "140" @default.
• W2023790596 startingPage "129" @default.
• W2023790596 abstract "Calculations are presented describing the influence of external diffusion in the kinetics of solid-phase immunoassays. The analysis is concerned with systems where one reactant is immobilized at the surface of a sphere of arbitrary radius. The solution for a plane surface is found as a limiting case. The factors determining whether the reaction is diffusion or reaction controlled are found to be sphere radius, surface concentration of binding sites, forward reaction rate and diffusion constant of reacting species. Means of determining whether the reaction is diffusion or reaction controlled from observable quantities are described. When applied to heterogeneous antibody-antigen binding it is found that normally the binding to cell-size spheres is not limited by external diffusion. However, when applied to solid-phase assays with high surface concentrations of binding sites immobilized at plane surfaces or macroscopic spheres the binding is found to be diffusion limited. The importance of a mass transfer analysis in this case is also discussed." @default.
• W2023790596 created "2016-06-24" @default.
• W2023790596 creator A5005224774 @default.
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• W2023790596 date "1986-05-01" @default.
• W2023790596 modified "2023-10-17" @default.
• W2023790596 title "External diffusion in solid-phase immunoassays" @default.
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• W2023790596 doi "https://doi.org/10.1016/s0022-5193(86)80169-2" @default.
• W2023790596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3537530" @default.
• W2023790596 hasPublicationYear "1986" @default.
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