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- W2023792806 abstract "Durch Derepression wird bei Hefezellen vor allem die Aktivität der sauren Phosphatase erhöht. Dieses Enzym war kinetisch nur auf der Zelloberfläche nachzuweisen. Im Elektronenmikroskop konnten histochemisch die saure Phosphatase in der äuβeren Schicht des Zellwalles lokalisiert werden. Darüber hinaus konnte die saure Phosphatase elektronenmikroskopisch auch im Zellinnern nachgewiesen werden. Sie ist hier an lysosomenähnliche Granula gebunden, deren Inhalt nach auβen abgegeben wird. Nach Repression fanden sich die Bleiphosphatniederschläge nur noch vereinzelt an der äuβeren Schicht des Zellwalles. Auch die Zahl der lysosomenähnlichen Granula und ihr Enzymgehalt nahmen ab. The activity of acid phosphatase in yeast cells is increased by derepression. The enzyme was only to be found kinetically on the cell surface. In the electron microscope acid phosphatase were localized histochemically in the external layer of the cell wall. Furthermore acid phosphatase was also shown in the cytoplasma. Here the enzyme is bound to granules resembling lysosomes. The content of the granules is released into the extracellular space. After repression only few lead phosphate precipitations are found in the external layer of the cell wall. The number of lysosome-like particles and their enzymatic activity is decreased." @default.
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- W2023792806 date "1967-01-01" @default.
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- W2023792806 title "Über das Verhalten und die Lokalisation der sauren Phosphatase von Hefezellen bei Repression und Derepression" @default.
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- W2023792806 doi "https://doi.org/10.1016/0014-4827(67)90118-8" @default.
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