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• W2023797303 startingPage "179" @default.
• W2023797303 abstract "A sensitive and accurate solid-phase assay for the quantitative determination of hyaluronic acid (HA) is described. The wells of the polystyrene microplates used were coated with glutaraldehyde followed, via a Schiff's base bond, with spermine to introduce amino groups. HA was added to the activated microwells in the presence of carbodiimide and left to bind via a peptide bond to the amino groups. Then aggrecan solution was added to the wells of the microtiter plates to interact with its G1 domain with hyaluronic acid, and the amounts of aggrecan bound were measured immunochemically. The inhibition of the binding between aggrecan and immobilized HA due to soluble HA present in reference solutions showed linearity in the range of concentrations 0.1 to 0.7 microg/ml. The reaction is specific and rapid and can be widely used for the calculation of HA in body fluids directly and in tissue samples after a brief digestion with a proteolytic enzyme." @default.
• W2023797303 created "2016-06-24" @default.
• W2023797303 creator A5004487484 @default.
• W2023797303 creator A5030413297 @default.
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• W2023797303 date "2003-09-01" @default.
• W2023797303 modified "2023-10-18" @default.
• W2023797303 title "A solid-phase assay for the quantitative analysis of hyaluronic acid at the nanogram level" @default.
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• W2023797303 doi "https://doi.org/10.1016/s0003-2697(03)00386-5" @default.
• W2023797303 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12927822" @default.
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