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• W2023809334 endingPage "218" @default.
• W2023809334 startingPage "208" @default.
• W2023809334 abstract "The nonstructural protein 3 (NS3) of Dengue virus (DV) is a multifunctional enzyme carrying activities involved in viral RNA replication and capping: helicase, nucleoside 5'-triphosphatase (NTPase), and RNA 5'-triphosphatase (RTPase). Here, a 54-kDa C-terminal domain of NS3 (DeltaNS3) bearing all three activities was expressed as a recombinant protein. Structure-based sequence analysis in comparison with Hepatitis C virus (HCV) helicase indicates the presence of a HCV-helicase-like catalytic core domain in the N-terminal part of DeltaNS3, whereas the C-terminal part seems to be different. In this report, we show that the RTPase activity of DeltaNS3 is Mg2+-dependent as are both helicase and NTPase activities. Mutational analysis shows that the RTPase activity requires an intact NTPase/helicase Walker B motif in the helicase core, consistent with the fact that such motifs are involved in the coordination of Mg2+. The R513A substitution in the C-terminal domain of DeltaNS3 abrogates helicase activity and strongly diminishes RTPase activity, indicating that both activities are functionally coupled. DV RTPase seems to belong to a new class of Mg2+-dependent RTPases, which use the active center of the helicase/NTPase catalytic core in conjunction with elements in the C-terminal domain." @default.
• W2023809334 created "2016-06-24" @default.
• W2023809334 creator A5003168652 @default.
• W2023809334 creator A5039783946 @default.
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• W2023809334 creator A5071436463 @default.
• W2023809334 creator A5072047303 @default.
• W2023809334 creator A5090204935 @default.
• W2023809334 date "2004-10-01" @default.
• W2023809334 modified "2023-10-06" @default.
• W2023809334 title "The RNA helicase, nucleotide 5′-triphosphatase, and RNA 5′-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic core" @default.
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• W2023809334 doi "https://doi.org/10.1016/j.virol.2004.07.004" @default.
• W2023809334 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15464841" @default.
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