FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023821611> ?p ?o ?g. }

• W2023821611 endingPage "1842" @default.
• W2023821611 startingPage "1828" @default.
• W2023821611 abstract "Thermosensitive transient receptor potential (TRP) proteins such as TRPV1-TRPV4 are all heat-activated non-selective cation channels that are modestly permeable to Ca(2+). TRPV1, TRPV3, and TRPV4 functional expression were previously identified in human corneal epithelial cells (HCEC). However, the membrane currents were not described underlying their activation by either selective agonists or thermal variation. This study characterized the membrane currents and [Ca(2+)](i) transients induced by thermal and agonist TRPV1 and 4 stimulation. TRPV1 and 4 expressions were confirmed by RT-PCR and TRPV2 transcripts were also detected. In fura2-loaded HCEC, a TRPV1-3 selective agonist, 100 µM 2-aminoethoxydiphenyl borate (2-APB), induced intracellular Ca(2+) transients and an increase in non-selective cation outward currents that were suppressed by ruthenium-red (RuR) (10-20 µM), a non-selective TRPV channel blocker. These changes were also elicited by rises in ambient temperature from 25 to over 40 °C. RuR (5 µM) and a selective TRPV1 channel blocker capsazepine CPZ (10 µM) or another related blocker, lanthanum chloride (La(3+)) (100 µM) suppressed these temperature-induced Ca(2+) increases. Planar patch-clamp technique was used to characterize the currents underlying Ca(2+) transients. Increasing the temperature to over 40 °C induced reversible rises in non-selective cation currents. Moreover, a hypotonic challenge (25%) increased non-selective cation currents confirming TRPV4 activity. We conclude that HCEC possess in addition to thermosensitive TRPV3 activity TRPV1, TRPV2, and TRPV4 activity. Their activation confers temperature sensitivity at the ocular surface, which may protect the cornea against such stress." @default.
• W2023821611 created "2016-06-24" @default.
• W2023821611 creator A5028940174 @default.
• W2023821611 creator A5035130589 @default.
• W2023821611 creator A5059499344 @default.
• W2023821611 creator A5065123820 @default.
• W2023821611 creator A5076607475 @default.
• W2023821611 creator A5086161688 @default.
• W2023821611 date "2011-04-19" @default.
• W2023821611 modified "2023-10-05" @default.
• W2023821611 title "Thermosensitive transient receptor potential channels in human corneal epithelial cells" @default.
• W2023821611 cites W1554059507 @default.
• W2023821611 cites W1707717597 @default.
• W2023821611 cites W1836265445 @default.
• W2023821611 cites W1923918181 @default.
• W2023821611 cites W1967621441 @default.
• W2023821611 cites W1967881723 @default.
• W2023821611 cites W1970629236 @default.
• W2023821611 cites W1973977506 @default.
• W2023821611 cites W1974058597 @default.
• W2023821611 cites W1976307713 @default.
• W2023821611 cites W1976919646 @default.
• W2023821611 cites W1986073598 @default.
• W2023821611 cites W1995330222 @default.
• W2023821611 cites W1997328210 @default.
• W2023821611 cites W2001426137 @default.
• W2023821611 cites W2003336901 @default.
• W2023821611 cites W2004925076 @default.
• W2023821611 cites W2007803500 @default.
• W2023821611 cites W2008406257 @default.
• W2023821611 cites W2009967312 @default.
• W2023821611 cites W2011631371 @default.
• W2023821611 cites W2012577197 @default.
• W2023821611 cites W2015498999 @default.
• W2023821611 cites W2022262895 @default.
• W2023821611 cites W2022429901 @default.
• W2023821611 cites W2027265533 @default.
• W2023821611 cites W2035348433 @default.
• W2023821611 cites W2035870964 @default.
• W2023821611 cites W2036179916 @default.
• W2023821611 cites W2038275562 @default.
• W2023821611 cites W2041322673 @default.
• W2023821611 cites W2042850894 @default.
• W2023821611 cites W2045459703 @default.
• W2023821611 cites W2051613742 @default.
• W2023821611 cites W2052482154 @default.
• W2023821611 cites W2063819509 @default.
• W2023821611 cites W2066260339 @default.
• W2023821611 cites W2077113145 @default.
• W2023821611 cites W2084590250 @default.
• W2023821611 cites W2089531185 @default.
• W2023821611 cites W2090617423 @default.
• W2023821611 cites W2092156066 @default.
• W2023821611 cites W2097824035 @default.
• W2023821611 cites W2101242815 @default.
• W2023821611 cites W2102759708 @default.
• W2023821611 cites W2104721296 @default.
• W2023821611 cites W2106916570 @default.
• W2023821611 cites W2107057154 @default.
• W2023821611 cites W2114908816 @default.
• W2023821611 cites W2119355019 @default.
• W2023821611 cites W2126403388 @default.
• W2023821611 cites W2127012396 @default.
• W2023821611 cites W2128932427 @default.
• W2023821611 cites W2129020034 @default.
• W2023821611 cites W2131716718 @default.
• W2023821611 cites W2137224035 @default.
• W2023821611 cites W2140880966 @default.
• W2023821611 cites W2141260882 @default.
• W2023821611 cites W2144888928 @default.
• W2023821611 cites W2149429155 @default.
• W2023821611 cites W2152585394 @default.
• W2023821611 cites W2172000561 @default.
• W2023821611 cites W2342304873 @default.
• W2023821611 cites W40623430 @default.
• W2023821611 cites W4233595534 @default.
• W2023821611 cites W68622123 @default.
• W2023821611 doi "https://doi.org/10.1002/jcp.22514" @default.
• W2023821611 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3072442" @default.
• W2023821611 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21506114" @default.
• W2023821611 hasPublicationYear "2011" @default.
• W2023821611 type Work @default.
• W2023821611 sameAs 2023821611 @default.
• W2023821611 citedByCount "48" @default.
• W2023821611 countsByYear W20238216112012 @default.
• W2023821611 countsByYear W20238216112013 @default.
• W2023821611 countsByYear W20238216112014 @default.
• W2023821611 countsByYear W20238216112015 @default.
• W2023821611 countsByYear W20238216112016 @default.
• W2023821611 countsByYear W20238216112017 @default.
• W2023821611 countsByYear W20238216112018 @default.
• W2023821611 countsByYear W20238216112019 @default.
• W2023821611 countsByYear W20238216112020 @default.
• W2023821611 countsByYear W20238216112021 @default.
• W2023821611 countsByYear W20238216112022 @default.
• W2023821611 countsByYear W20238216112023 @default.
• W2023821611 crossrefType "journal-article" @default.
• W2023821611 hasAuthorship W2023821611A5028940174 @default.

• next