Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023842706> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2023842706 endingPage "8260" @default.
- W2023842706 startingPage "8254" @default.
- W2023842706 abstract "When cytochrome c oxidase is incubated at 43 degrees C for approximately 75 min in a solution containing the zwitterionic detergent sulfobetaine 12, the CuA site is converted into a type II copper as judged by changes in the 830-nm absorption band and the EPR spectrum of the enzyme. SDS-PAGE and sucrose gradient ultracentrifugation indicate concomitant loss of subunit III and monomerization of the enzyme during the heat treatment. Comparison of the optical and resonance Raman spectra of the heat-treated and native protein shows that the heme chromophores are not significantly perturbed; the resonance Raman data indicate that the small heme perturbations observed are limited to the cytochrome a3 site. Proton pumping measurements, conducted on the modified enzyme reconstituted into phospholipid vesicles, indicate that these vesicles are unusually permeable toward protons during turnover, as previously reported for the p-(hydroxymercuri)benzoate-modified oxidase and the modified enzyme obtained by heat treatment in lauryl maltoside. The sulfobetaine 12 modified enzyme is no longer capable of undergoing the recently reported conformational transition in which the tryptophan fluorescence changes upon reduction of the low-potential metal centers. Control studies on the monomeric and subunit III dissociated enzymes suggest that the disruption of this conformational change in the heat-treated oxidase is most likely associated with perturbation of the CuA site. These results lend support to the suggestion that the fluorescence-monitored conformational change of the native enzyme is initiated by reduction of the CuA site [Copeland et al. (1987) Biochemistry 26, 7311]." @default.
- W2023842706 created "2016-06-24" @default.
- W2023842706 creator A5017230178 @default.
- W2023842706 creator A5053167046 @default.
- W2023842706 creator A5057044415 @default.
- W2023842706 creator A5067466789 @default.
- W2023842706 date "1988-10-18" @default.
- W2023842706 modified "2023-10-14" @default.
- W2023842706 title "Conversion of CuA to a type II copper in cytochrome c oxidase" @default.
- W2023842706 cites W1517540538 @default.
- W2023842706 cites W1542250359 @default.
- W2023842706 cites W1544518693 @default.
- W2023842706 cites W1544944356 @default.
- W2023842706 cites W1557071567 @default.
- W2023842706 cites W1568687764 @default.
- W2023842706 cites W1761464280 @default.
- W2023842706 cites W1964180674 @default.
- W2023842706 cites W1987889272 @default.
- W2023842706 cites W1995826358 @default.
- W2023842706 cites W1999957017 @default.
- W2023842706 cites W2001345051 @default.
- W2023842706 cites W2018847244 @default.
- W2023842706 cites W2019030231 @default.
- W2023842706 cites W2019789717 @default.
- W2023842706 cites W2026359797 @default.
- W2023842706 cites W2030910868 @default.
- W2023842706 cites W2031904472 @default.
- W2023842706 cites W2032136501 @default.
- W2023842706 cites W2041957158 @default.
- W2023842706 cites W2054121272 @default.
- W2023842706 cites W2054167141 @default.
- W2023842706 cites W2063661315 @default.
- W2023842706 cites W2069360700 @default.
- W2023842706 cites W2070144421 @default.
- W2023842706 cites W2092481009 @default.
- W2023842706 cites W2156274644 @default.
- W2023842706 cites W2950213309 @default.
- W2023842706 cites W978966305 @default.
- W2023842706 cites W2074850355 @default.
- W2023842706 doi "https://doi.org/10.1021/bi00421a040" @default.
- W2023842706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2852958" @default.
- W2023842706 hasPublicationYear "1988" @default.
- W2023842706 type Work @default.
- W2023842706 sameAs 2023842706 @default.
- W2023842706 citedByCount "12" @default.
- W2023842706 countsByYear W20238427062014 @default.
- W2023842706 countsByYear W20238427062022 @default.
- W2023842706 crossrefType "journal-article" @default.
- W2023842706 hasAuthorship W2023842706A5017230178 @default.
- W2023842706 hasAuthorship W2023842706A5053167046 @default.
- W2023842706 hasAuthorship W2023842706A5057044415 @default.
- W2023842706 hasAuthorship W2023842706A5067466789 @default.
- W2023842706 hasConcept C121332964 @default.
- W2023842706 hasConcept C123249941 @default.
- W2023842706 hasConcept C181199279 @default.
- W2023842706 hasConcept C185592680 @default.
- W2023842706 hasConcept C187961010 @default.
- W2023842706 hasConcept C2776217839 @default.
- W2023842706 hasConcept C2777339483 @default.
- W2023842706 hasConcept C2780265247 @default.
- W2023842706 hasConcept C2909484822 @default.
- W2023842706 hasConcept C46141821 @default.
- W2023842706 hasConcept C55493867 @default.
- W2023842706 hasConcept C71240020 @default.
- W2023842706 hasConcept C75473681 @default.
- W2023842706 hasConceptScore W2023842706C121332964 @default.
- W2023842706 hasConceptScore W2023842706C123249941 @default.
- W2023842706 hasConceptScore W2023842706C181199279 @default.
- W2023842706 hasConceptScore W2023842706C185592680 @default.
- W2023842706 hasConceptScore W2023842706C187961010 @default.
- W2023842706 hasConceptScore W2023842706C2776217839 @default.
- W2023842706 hasConceptScore W2023842706C2777339483 @default.
- W2023842706 hasConceptScore W2023842706C2780265247 @default.
- W2023842706 hasConceptScore W2023842706C2909484822 @default.
- W2023842706 hasConceptScore W2023842706C46141821 @default.
- W2023842706 hasConceptScore W2023842706C55493867 @default.
- W2023842706 hasConceptScore W2023842706C71240020 @default.
- W2023842706 hasConceptScore W2023842706C75473681 @default.
- W2023842706 hasIssue "21" @default.
- W2023842706 hasLocation W20238427061 @default.
- W2023842706 hasLocation W20238427062 @default.
- W2023842706 hasOpenAccess W2023842706 @default.
- W2023842706 hasPrimaryLocation W20238427061 @default.
- W2023842706 hasRelatedWork W1031849976 @default.
- W2023842706 hasRelatedWork W1971246384 @default.
- W2023842706 hasRelatedWork W2039837927 @default.
- W2023842706 hasRelatedWork W2042109491 @default.
- W2023842706 hasRelatedWork W2060749628 @default.
- W2023842706 hasRelatedWork W2063289130 @default.
- W2023842706 hasRelatedWork W2077201291 @default.
- W2023842706 hasRelatedWork W2079176793 @default.
- W2023842706 hasRelatedWork W2160213712 @default.
- W2023842706 hasRelatedWork W2176282060 @default.
- W2023842706 hasVolume "27" @default.
- W2023842706 isParatext "false" @default.
- W2023842706 isRetracted "false" @default.
- W2023842706 magId "2023842706" @default.
- W2023842706 workType "article" @default.