FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023861301> ?p ?o ?g. }

• W2023861301 endingPage "1092" @default.
• W2023861301 startingPage "1086" @default.
• W2023861301 abstract "ObjectiveTo examine the effect of interleukin-6 (IL-6) on estrogen production and aromatase activity using a human granulosa tumor cell line (KGN cells). The involvement of the mitogen-activated protein kinase (MAPK) cascade in the inhibitory effects of IL-6 on estrogen production was also evaluated.DesignMolecular and biological studies of KGN cells.SettingDepartment of Obstetrics and Gynecology, Tottori University Hospital, Yonago, Japan.Main Outcome Measure(s)Gene expression of IL-6 and the IL-6 receptor was analyzed by reverse transcription–polymerase chain reaction and Southern blot analysis. KGN cells were cultured for 48 hours with IL-6 (0.1–10 ng/mL) or IL-6 (10 ng/mL) plus a mitogen activated protein kinase-extracellular signal regulated kinase kinase 1/2 (MEK1/2) inhibitor U0126 (10 μM). Estradiol concentration in the culture supernatants was measured by means of enzyme immunoassay, [1β-3H] androstenedione was added to the cell lysate supernatant, and aromatase activity was determined by measuring the amount of [3H] H2O released upon the conversion of [1β-3H] androstenedione to estrone. To examine the activation of intracellular signal transduction molecules induced by IL-6, the phosphorylation of Stat3, p38 MAPK, and extracellular signal-regulated kinase 1/2 (ERK1/2) was examined by Western blotting.Result(s)Gene expression of IL-6 and its receptor was detected in KGN cells. Estradiol secretion was significantly inhibited by adding IL-6, which also suppressed aromatase activity to 50% of the control. In addition, pretreatment with U0126 restored the IL-6–induced suppression of aromatase activity. IL-6 markedly enhanced the phosphorylation of ERK1/2, but not Stat3 and p38 MAPK. U0126 markedly reduced the level of the IL-6–induced phosphorylation of ERK1/2.Conclusion(s)These findings demonstrate that IL-6 may reduce estrogen production via the MAPK signal pathway in human granulosa cells. The results may support the notion that IL-6 is related to impaired estrogen biosynthesis in patients with endometriosis. To examine the effect of interleukin-6 (IL-6) on estrogen production and aromatase activity using a human granulosa tumor cell line (KGN cells). The involvement of the mitogen-activated protein kinase (MAPK) cascade in the inhibitory effects of IL-6 on estrogen production was also evaluated. Molecular and biological studies of KGN cells. Department of Obstetrics and Gynecology, Tottori University Hospital, Yonago, Japan. Gene expression of IL-6 and the IL-6 receptor was analyzed by reverse transcription–polymerase chain reaction and Southern blot analysis. KGN cells were cultured for 48 hours with IL-6 (0.1–10 ng/mL) or IL-6 (10 ng/mL) plus a mitogen activated protein kinase-extracellular signal regulated kinase kinase 1/2 (MEK1/2) inhibitor U0126 (10 μM). Estradiol concentration in the culture supernatants was measured by means of enzyme immunoassay, [1β-3H] androstenedione was added to the cell lysate supernatant, and aromatase activity was determined by measuring the amount of [3H] H2O released upon the conversion of [1β-3H] androstenedione to estrone. To examine the activation of intracellular signal transduction molecules induced by IL-6, the phosphorylation of Stat3, p38 MAPK, and extracellular signal-regulated kinase 1/2 (ERK1/2) was examined by Western blotting. Gene expression of IL-6 and its receptor was detected in KGN cells. Estradiol secretion was significantly inhibited by adding IL-6, which also suppressed aromatase activity to 50% of the control. In addition, pretreatment with U0126 restored the IL-6–induced suppression of aromatase activity. IL-6 markedly enhanced the phosphorylation of ERK1/2, but not Stat3 and p38 MAPK. U0126 markedly reduced the level of the IL-6–induced phosphorylation of ERK1/2. These findings demonstrate that IL-6 may reduce estrogen production via the MAPK signal pathway in human granulosa cells. The results may support the notion that IL-6 is related to impaired estrogen biosynthesis in patients with endometriosis." @default.
• W2023861301 created "2016-06-24" @default.
• W2023861301 creator A5017238643 @default.
• W2023861301 creator A5028337478 @default.
• W2023861301 creator A5033028239 @default.
• W2023861301 creator A5049926214 @default.
• W2023861301 creator A5066723701 @default.
• W2023861301 creator A5076556711 @default.
• W2023861301 date "2005-04-01" @default.
• W2023861301 modified "2023-09-25" @default.
• W2023861301 title "Reduction of estrogen production by interleukin-6 in a human granulosa tumor cell line may have implications for endometriosis-associated infertility" @default.
• W2023861301 cites W1503307145 @default.
• W2023861301 cites W1979623508 @default.
• W2023861301 cites W2002196249 @default.
• W2023861301 cites W2019843041 @default.
• W2023861301 cites W2027572168 @default.
• W2023861301 cites W2034307696 @default.
• W2023861301 cites W2038002928 @default.
• W2023861301 cites W2040238312 @default.
• W2023861301 cites W2048198010 @default.
• W2023861301 cites W2049657573 @default.
• W2023861301 cites W2053211648 @default.
• W2023861301 cites W2054749660 @default.
• W2023861301 cites W2063147985 @default.
• W2023861301 cites W2085523985 @default.
• W2023861301 cites W2097611363 @default.
• W2023861301 cites W2106876536 @default.
• W2023861301 cites W2106939519 @default.
• W2023861301 cites W2266060285 @default.
• W2023861301 cites W2307404619 @default.
• W2023861301 cites W2405709589 @default.
• W2023861301 cites W2412222117 @default.
• W2023861301 cites W2415597826 @default.
• W2023861301 cites W2426778538 @default.
• W2023861301 cites W4245868295 @default.
• W2023861301 cites W4293247451 @default.
• W2023861301 doi "https://doi.org/10.1016/j.fertnstert.2004.12.014" @default.
• W2023861301 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15831279" @default.
• W2023861301 hasPublicationYear "2005" @default.
• W2023861301 type Work @default.
• W2023861301 sameAs 2023861301 @default.
• W2023861301 citedByCount "41" @default.
• W2023861301 countsByYear W20238613012013 @default.
• W2023861301 countsByYear W20238613012014 @default.
• W2023861301 countsByYear W20238613012015 @default.
• W2023861301 countsByYear W20238613012016 @default.
• W2023861301 countsByYear W20238613012017 @default.
• W2023861301 countsByYear W20238613012018 @default.
• W2023861301 countsByYear W20238613012019 @default.
• W2023861301 countsByYear W20238613012020 @default.
• W2023861301 countsByYear W20238613012021 @default.
• W2023861301 countsByYear W20238613012022 @default.
• W2023861301 countsByYear W20238613012023 @default.
• W2023861301 crossrefType "journal-article" @default.
• W2023861301 hasAuthorship W2023861301A5017238643 @default.
• W2023861301 hasAuthorship W2023861301A5028337478 @default.
• W2023861301 hasAuthorship W2023861301A5033028239 @default.
• W2023861301 hasAuthorship W2023861301A5049926214 @default.
• W2023861301 hasAuthorship W2023861301A5066723701 @default.
• W2023861301 hasAuthorship W2023861301A5076556711 @default.
• W2023861301 hasBestOaLocation W20238613011 @default.
• W2023861301 hasConcept C121608353 @default.
• W2023861301 hasConcept C126322002 @default.
• W2023861301 hasConcept C134018914 @default.
• W2023861301 hasConcept C153911025 @default.
• W2023861301 hasConcept C184235292 @default.
• W2023861301 hasConcept C185592680 @default.
• W2023861301 hasConcept C2776166826 @default.
• W2023861301 hasConcept C530470458 @default.
• W2023861301 hasConcept C57074206 @default.
• W2023861301 hasConcept C71924100 @default.
• W2023861301 hasConcept C86803240 @default.
• W2023861301 hasConcept C95444343 @default.
• W2023861301 hasConcept C97029542 @default.
• W2023861301 hasConceptScore W2023861301C121608353 @default.
• W2023861301 hasConceptScore W2023861301C126322002 @default.
• W2023861301 hasConceptScore W2023861301C134018914 @default.
• W2023861301 hasConceptScore W2023861301C153911025 @default.
• W2023861301 hasConceptScore W2023861301C184235292 @default.
• W2023861301 hasConceptScore W2023861301C185592680 @default.
• W2023861301 hasConceptScore W2023861301C2776166826 @default.
• W2023861301 hasConceptScore W2023861301C530470458 @default.
• W2023861301 hasConceptScore W2023861301C57074206 @default.
• W2023861301 hasConceptScore W2023861301C71924100 @default.
• W2023861301 hasConceptScore W2023861301C86803240 @default.
• W2023861301 hasConceptScore W2023861301C95444343 @default.
• W2023861301 hasConceptScore W2023861301C97029542 @default.
• W2023861301 hasIssue "4" @default.
• W2023861301 hasLocation W20238613011 @default.
• W2023861301 hasLocation W20238613012 @default.
• W2023861301 hasLocation W20238613013 @default.
• W2023861301 hasOpenAccess W2023861301 @default.
• W2023861301 hasPrimaryLocation W20238613011 @default.
• W2023861301 hasRelatedWork W1989693389 @default.
• W2023861301 hasRelatedWork W2009015955 @default.
• W2023861301 hasRelatedWork W2045679033 @default.
• W2023861301 hasRelatedWork W2054700953 @default.
• W2023861301 hasRelatedWork W2063998103 @default.

• next