Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023863634> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2023863634 endingPage "80" @default.
- W2023863634 startingPage "74" @default.
- W2023863634 abstract "Pyrraline (epsilon-2-(formyl-5-hydroxymethyl-pyrrol-1-yl)-L-norleucine) is an advanced Maillard reaction product formed from 3-deoxyglucosone in the non-enzymatic reaction between glucose and the epsilon-amino group of lysine residues on proteins. Although its presence in vivo as well as in in vitro incubations of proteins with sugars has been documented by immunochemical methods using polyclonal and monoclonal antibodies, its formation in proteins has recently been questioned by similar methodology. To clarify this issue, we investigated pyrraline formation in proteins following alkaline hydrolysis and quantitation by high-performance liquid chromatography on a C18 reverse-phase column. Time- and sugar concentration-dependent increase in pyrraline formation was noted in serum albumin incubated with either 100 mM glucose or 50 mM 3-deoxyglucosone. Formation of pyrraline from 3-deoxyglucosone was rapid at slightly acidic pH, confirming its synthetic pathway through this Maillard reaction intermediate. Low levels of pyrraline (< 10 pmol/mg protein) were also detected in a pool of human skin collagen by this method, but no age effect was apparent. Using a slightly different approach, pyrraline-like material was detected in human plasma proteins following enzyme digestion and analysis by high performance liquid chromatography. Plasma from diabetic patients showed a significant increase in pyrraline-like material compared to controls. The levels in diabetic and normal individuals were 21.6 +/- 9.56 and 12.8 +/- 5.6 pmol per mg protein, respectively (P = 0.005), reflecting thereby the elevated levels of the immediate precursor of pyrraline, 3-deoxyglucosone, in diabetic plasma." @default.
- W2023863634 created "2016-06-24" @default.
- W2023863634 creator A5048439423 @default.
- W2023863634 creator A5050412857 @default.
- W2023863634 creator A5075089513 @default.
- W2023863634 date "1995-02-01" @default.
- W2023863634 modified "2023-10-15" @default.
- W2023863634 title "Chromatographic evidence for pyrraline formation during protein glycation in vitro and in vivo" @default.
- W2023863634 cites W1493293629 @default.
- W2023863634 cites W1511235377 @default.
- W2023863634 cites W1676132058 @default.
- W2023863634 cites W1676406316 @default.
- W2023863634 cites W1968348609 @default.
- W2023863634 cites W1969779705 @default.
- W2023863634 cites W1973251040 @default.
- W2023863634 cites W1992592000 @default.
- W2023863634 cites W1996337164 @default.
- W2023863634 cites W1996459255 @default.
- W2023863634 cites W1998498936 @default.
- W2023863634 cites W2000896479 @default.
- W2023863634 cites W2001348757 @default.
- W2023863634 cites W2002485335 @default.
- W2023863634 cites W2016119603 @default.
- W2023863634 cites W2033101389 @default.
- W2023863634 cites W2077777115 @default.
- W2023863634 cites W2087024548 @default.
- W2023863634 cites W2094803517 @default.
- W2023863634 cites W2438873404 @default.
- W2023863634 cites W4229571803 @default.
- W2023863634 doi "https://doi.org/10.1016/0167-4838(94)00209-y" @default.
- W2023863634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7873594" @default.
- W2023863634 hasPublicationYear "1995" @default.
- W2023863634 type Work @default.
- W2023863634 sameAs 2023863634 @default.
- W2023863634 citedByCount "78" @default.
- W2023863634 countsByYear W20238636342012 @default.
- W2023863634 countsByYear W20238636342013 @default.
- W2023863634 countsByYear W20238636342015 @default.
- W2023863634 countsByYear W20238636342016 @default.
- W2023863634 countsByYear W20238636342017 @default.
- W2023863634 countsByYear W20238636342018 @default.
- W2023863634 countsByYear W20238636342019 @default.
- W2023863634 countsByYear W20238636342020 @default.
- W2023863634 countsByYear W20238636342021 @default.
- W2023863634 countsByYear W20238636342022 @default.
- W2023863634 countsByYear W20238636342023 @default.
- W2023863634 crossrefType "journal-article" @default.
- W2023863634 hasAuthorship W2023863634A5048439423 @default.
- W2023863634 hasAuthorship W2023863634A5050412857 @default.
- W2023863634 hasAuthorship W2023863634A5075089513 @default.
- W2023863634 hasConcept C146543888 @default.
- W2023863634 hasConcept C147990577 @default.
- W2023863634 hasConcept C170493617 @default.
- W2023863634 hasConcept C185592680 @default.
- W2023863634 hasConcept C2776997535 @default.
- W2023863634 hasConcept C2780758796 @default.
- W2023863634 hasConcept C35378473 @default.
- W2023863634 hasConcept C43617362 @default.
- W2023863634 hasConcept C55493867 @default.
- W2023863634 hasConceptScore W2023863634C146543888 @default.
- W2023863634 hasConceptScore W2023863634C147990577 @default.
- W2023863634 hasConceptScore W2023863634C170493617 @default.
- W2023863634 hasConceptScore W2023863634C185592680 @default.
- W2023863634 hasConceptScore W2023863634C2776997535 @default.
- W2023863634 hasConceptScore W2023863634C2780758796 @default.
- W2023863634 hasConceptScore W2023863634C35378473 @default.
- W2023863634 hasConceptScore W2023863634C43617362 @default.
- W2023863634 hasConceptScore W2023863634C55493867 @default.
- W2023863634 hasIssue "1" @default.
- W2023863634 hasLocation W20238636341 @default.
- W2023863634 hasLocation W20238636342 @default.
- W2023863634 hasOpenAccess W2023863634 @default.
- W2023863634 hasPrimaryLocation W20238636341 @default.
- W2023863634 hasRelatedWork W2008463780 @default.
- W2023863634 hasRelatedWork W2015871818 @default.
- W2023863634 hasRelatedWork W2023863634 @default.
- W2023863634 hasRelatedWork W2053622356 @default.
- W2023863634 hasRelatedWork W2058525009 @default.
- W2023863634 hasRelatedWork W2096528326 @default.
- W2023863634 hasRelatedWork W2138200625 @default.
- W2023863634 hasRelatedWork W2167259407 @default.
- W2023863634 hasRelatedWork W2182223333 @default.
- W2023863634 hasRelatedWork W3024383408 @default.
- W2023863634 hasVolume "1247" @default.
- W2023863634 isParatext "false" @default.
- W2023863634 isRetracted "false" @default.
- W2023863634 magId "2023863634" @default.
- W2023863634 workType "article" @default.