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- W2023871282 abstract "We have used an experimental arrangement comprising two photomultipliers and time-correlated single photon counting (TCSPC) detection to measure time and polarization-resolved fluorescence decays and images simultaneously. Polarization-resolved measurements can provide information which may be difficult to extract from lifetime measurements alone. The combination of fluorescence lifetime and time-resolved anisotropy in an imaging modality with two detectors minimizes the errors arising from bleaching of a sample between consecutive measurements. Anisotropy measurements can provide evidence of fluorescence resonance energy transfer between chemically identical fluorophores (homo-FRET). This phenomenon is not detectable in spectral or lifetime changes, yet a lowering of the anisotropy and a faster anisotropy decay can provide evidence for close proximity (≤ 10 nm) of adjacent fluorophores including dimerization and oligomerization of molecules. We have used FLIM and fluorescence anisotropy to measure variations in fluorescence lifetimes and anisotropy of GFP-tagged proteins in cells in immunological synapse samples and also acquire images of BODIPY-stained carcinoma cells." @default.
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- W2023871282 date "2008-02-07" @default.
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- W2023871282 title "Multidimensional multiphoton fluorescence lifetime imaging of cells" @default.
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