Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023872861> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2023872861 abstract "Cell adhesion through integrin receptors to the extracellular matrix is essential for pattern formation during tissue morphogenesis. For example, cell adhesion differences correlate with epithelial branching, and stem cells often reside in microenvironments essential for stem cell maintenance and differentiation. Tissue engineering relies on the ability to replicate in vivo pattern formation to grow complex multicellular tissues in vitro, a largely unresolved challenge. Although cell growth can be patterned by immobilizing matrix proteins by photolithography or microcontact printing, such techniques are not easily adaptable to a physiological 3D environment and most importantly do not allow dynamic control of cell adhesion during in vitro organogenesis. To address this challenge, we started to develop a system by which cell-matrix interactions can be reversibly modulated by patterned 470 nm illumination utilizing a novel short peptide, Zdk, that binds to the dark-state of oat LOV2 with high affinity. Aminosilane-coated coverslips were reacted with NHS-PEG-biotin conjugates, and a biotinylated LOV2 domain attached through neutravidin. As proof-of-principle, we bound mCherry-Zdk-RGD fusion proteins to biotin-LOV2-coated coverslips, and demonstrate rapid, reversible and patterned light-induced release of mCherry-Zdk-RGD from the surface. We are investigating how light-triggered release of cell matrix attachment controls focal adhesion dynamics and cell migration. Rapid rebinding of mCherry-Zdk-RGD is due to the photochemical properties of LOV2, which rapidly reverts to the dark state after photoactivation. Because this may be too fast to manipulate cell-matrix adhesions that turn over at a substantially slower timescale, we carried out a mutagenesis screen of LOV2 variants generated by error-prone PCR. Using fluorescence recovery of the LOV2-bound flavin molecule as readout, we identified numerous novel mutations near the flavin-binding site that revert to the dark state at much slower rates, presumably by stabilizing the flavin-cysteinyl adduct." @default.
- W2023872861 created "2016-06-24" @default.
- W2023872861 creator A5026554721 @default.
- W2023872861 creator A5039591558 @default.
- W2023872861 date "2014-01-01" @default.
- W2023872861 modified "2023-09-28" @default.
- W2023872861 title "Light-Modulated Cell Adhesion to Control Cell and Tissue Morphogenesis" @default.
- W2023872861 doi "https://doi.org/10.1016/j.bpj.2013.11.4325" @default.
- W2023872861 hasPublicationYear "2014" @default.
- W2023872861 type Work @default.
- W2023872861 sameAs 2023872861 @default.
- W2023872861 citedByCount "0" @default.
- W2023872861 crossrefType "journal-article" @default.
- W2023872861 hasAuthorship W2023872861A5026554721 @default.
- W2023872861 hasAuthorship W2023872861A5039591558 @default.
- W2023872861 hasBestOaLocation W20238728611 @default.
- W2023872861 hasConcept C104317684 @default.
- W2023872861 hasConcept C12554922 @default.
- W2023872861 hasConcept C137738243 @default.
- W2023872861 hasConcept C142613039 @default.
- W2023872861 hasConcept C1491633281 @default.
- W2023872861 hasConcept C178790620 @default.
- W2023872861 hasConcept C185592680 @default.
- W2023872861 hasConcept C189165786 @default.
- W2023872861 hasConcept C195687474 @default.
- W2023872861 hasConcept C2779260011 @default.
- W2023872861 hasConcept C40692019 @default.
- W2023872861 hasConcept C55493867 @default.
- W2023872861 hasConcept C84416704 @default.
- W2023872861 hasConcept C85789140 @default.
- W2023872861 hasConcept C86803240 @default.
- W2023872861 hasConcept C95444343 @default.
- W2023872861 hasConceptScore W2023872861C104317684 @default.
- W2023872861 hasConceptScore W2023872861C12554922 @default.
- W2023872861 hasConceptScore W2023872861C137738243 @default.
- W2023872861 hasConceptScore W2023872861C142613039 @default.
- W2023872861 hasConceptScore W2023872861C1491633281 @default.
- W2023872861 hasConceptScore W2023872861C178790620 @default.
- W2023872861 hasConceptScore W2023872861C185592680 @default.
- W2023872861 hasConceptScore W2023872861C189165786 @default.
- W2023872861 hasConceptScore W2023872861C195687474 @default.
- W2023872861 hasConceptScore W2023872861C2779260011 @default.
- W2023872861 hasConceptScore W2023872861C40692019 @default.
- W2023872861 hasConceptScore W2023872861C55493867 @default.
- W2023872861 hasConceptScore W2023872861C84416704 @default.
- W2023872861 hasConceptScore W2023872861C85789140 @default.
- W2023872861 hasConceptScore W2023872861C86803240 @default.
- W2023872861 hasConceptScore W2023872861C95444343 @default.
- W2023872861 hasLocation W20238728611 @default.
- W2023872861 hasOpenAccess W2023872861 @default.
- W2023872861 hasPrimaryLocation W20238728611 @default.
- W2023872861 hasRelatedWork W1681548011 @default.
- W2023872861 hasRelatedWork W2001697939 @default.
- W2023872861 hasRelatedWork W2026932365 @default.
- W2023872861 hasRelatedWork W2044115084 @default.
- W2023872861 hasRelatedWork W2064541498 @default.
- W2023872861 hasRelatedWork W2093206589 @default.
- W2023872861 hasRelatedWork W2093691382 @default.
- W2023872861 hasRelatedWork W2107984108 @default.
- W2023872861 hasRelatedWork W2118251000 @default.
- W2023872861 hasRelatedWork W2119110546 @default.
- W2023872861 hasRelatedWork W2301088451 @default.
- W2023872861 hasRelatedWork W2402289513 @default.
- W2023872861 hasRelatedWork W2763733299 @default.
- W2023872861 hasRelatedWork W3124190827 @default.
- W2023872861 hasRelatedWork W3206517643 @default.
- W2023872861 hasRelatedWork W64565635 @default.
- W2023872861 hasRelatedWork W895043296 @default.
- W2023872861 hasRelatedWork W2198087359 @default.
- W2023872861 hasRelatedWork W2408044429 @default.
- W2023872861 hasRelatedWork W2809612607 @default.
- W2023872861 isParatext "false" @default.
- W2023872861 isRetracted "false" @default.
- W2023872861 magId "2023872861" @default.
- W2023872861 workType "article" @default.