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- W2023873218 abstract "A concentrate of mononuclear bone marrow cells is often desired for ex vivo treatment with pharmacologic agents, monoclonal antibodies, cytokines, and other agents prior to transplantation. A method has been developed for automated separation of mononuclear cells from large volumes of harvested bone marrow. A programmable instrument originally designed for clinical ex vivo cell separation and the plasma-pheresis of patients and blood donors was adapted to permit rapid preparation, in a closed sterile system, of a bone marrow product enriched with mononuclear cells. A mean (±/− SEM) of 53 ±/− 30 percent of the original mononuclear cells was recovered in a volume of 125 ±/− 42 mL containing 82 ±/− 12 percent mononuclear cells. This technique removed 95 ±/− 9 percent of the red cells in the original marrow. No density gradient materials or sedimenting agents were employed in this process. Of 36 marrows processed by this technique, 19 autologous (6 of which were purged with 4-hydroperoxycyclophosphamide) and 7 allogeneic marrows have been transplanted, with all evaluable patients achieving a neutrophil count of 0.5 × 10(9) per L in a mean (±/− SEM) of 21 ±/− 6 days." @default.
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- W2023873218 date "1991-10-01" @default.
- W2023873218 modified "2023-10-06" @default.
- W2023873218 title "Automated processing of human bone marrow can result in a population of mononuclear cells capable of achieving engraftment following transplantation" @default.
- W2023873218 doi "https://doi.org/10.1046/j.1537-2995.1991.31892023498.x" @default.
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