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- W2023874318 abstract "A short amino acid sequence coding for the mature Pseudomonas aeruginosa OprI lipoprotein was fused to a mini-Tn5 plasposon (mini-transposon with an origin of replication) with tetracycline resistance in order to generate in-frame fusion proteins after transposition. After conjugative transfer to Burkholderia multivorans, clones reacting with an anti-OprI mab were selected. In-frame OprI-tagged proteins were detected and identified for six clones. The six C-tagged proteins were detected by immunoblot. The different mutants had insertions into a histone H1-like coding gene, cspD, encoding a cold-shock protein, dsbC, encoding a putative outer membrane lipoprotein involved in thiol-disulfide exchange, paaE, a ferredoxin-NADPH reductase gene, a gene for the catabolism of propionate, and one encoding an unknown protein." @default.
- W2023874318 created "2016-06-24" @default.
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- W2023874318 date "2003-07-01" @default.
- W2023874318 modified "2023-10-14" @default.
- W2023874318 title "A new mini-transposon for in vivo protein epitope tagging: application to Burkholderia multivorans" @default.
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- W2023874318 doi "https://doi.org/10.1016/s0923-2508(03)00080-9" @default.
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