FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023880109> ?p ?o ?g. }

• W2023880109 endingPage "238" @default.
• W2023880109 startingPage "227" @default.
• W2023880109 abstract "Five mutant versions of aspartate transcarbamylase have been isolated, all with single amino acid substitutions in the catalytic chain of the enzyme. A previously isolated pyrB nonsense mutant was suppressed with supB, supC, supD and supG to create enzymes with glutamine, tyrosine, serine or lysine, respectively, inserted at the position of the nonsense codon. Each of these enzymes was purified to homogeneity and kinetically characterized. The approximate location of the substitution was determined by using tryptic fingerprints of the wild-type enzyme and the enzyme obtained with a tyrosine residue inserted at the position of the nonsense codon. By first cloning the pyrBI operon, from the original pyrB nonsense strain, followed by sequencing of the appropriate portion of the gene, the exact location of the mutation was determined to be at position 209 of the catalytic chain. Site-directed mutagenesis was used to generate versions of aspartate transcarbamylase with tyrosine and glutamic acid at this position. The Tyr209 enzyme is identical with that obtained by suppression of the original nonsense mutation with supC. The two enzymes produced by site-directed mutagenesis were purified using a newly created overproducing strain. Kinetic analysis revealed that each mutant has an altered affinity for aspartate, as judged by variations in the substrate concentration at one-half maximal activity. In addition, the mutants exhibit altered Hill coefficients and maximal activities. In the wild-type enzyme, position 209 is a tryptophan residue that is involved in the stabilization of a bend in the molecule near the subunit interface region. The alteration in homotropic co-operativity seems to be due to changes induced in this bend in the molecule, which stabilizes alternate conformational states of the enzyme." @default.
• W2023880109 created "2016-06-24" @default.
• W2023880109 creator A5048955712 @default.
• W2023880109 creator A5054810944 @default.
• W2023880109 creator A5057251750 @default.
• W2023880109 creator A5059952544 @default.
• W2023880109 creator A5066648363 @default.
• W2023880109 date "1986-05-01" @default.
• W2023880109 modified "2023-09-30" @default.
• W2023880109 title "Involvement of tryptophan 209 in the allosteric interactions of Escherichia coli aspartate transcarbamylase using single amino acid substitution mutants" @default.
• W2023880109 cites W1535308634 @default.
• W2023880109 cites W1545540883 @default.
• W2023880109 cites W1555002836 @default.
• W2023880109 cites W1561167748 @default.
• W2023880109 cites W1565023424 @default.
• W2023880109 cites W1568502048 @default.
• W2023880109 cites W1606830445 @default.
• W2023880109 cites W1775749144 @default.
• W2023880109 cites W184399086 @default.
• W2023880109 cites W1985937560 @default.
• W2023880109 cites W2004020018 @default.
• W2023880109 cites W2006535900 @default.
• W2023880109 cites W2009576399 @default.
• W2023880109 cites W2017296679 @default.
• W2023880109 cites W2018225090 @default.
• W2023880109 cites W2020498566 @default.
• W2023880109 cites W2020734449 @default.
• W2023880109 cites W2036518772 @default.
• W2023880109 cites W2042807617 @default.
• W2023880109 cites W2047595408 @default.
• W2023880109 cites W2068977571 @default.
• W2023880109 cites W2081479690 @default.
• W2023880109 cites W2084884863 @default.
• W2023880109 cites W2092301510 @default.
• W2023880109 cites W2097758310 @default.
• W2023880109 cites W2100837269 @default.
• W2023880109 cites W2111301002 @default.
• W2023880109 cites W2132068224 @default.
• W2023880109 cites W4249057392 @default.
• W2023880109 cites W4293247451 @default.
• W2023880109 cites W76089181 @default.
• W2023880109 doi "https://doi.org/10.1016/0022-2836(86)90393-1" @default.
• W2023880109 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3537306" @default.
• W2023880109 hasPublicationYear "1986" @default.
• W2023880109 type Work @default.
• W2023880109 sameAs 2023880109 @default.
• W2023880109 citedByCount "25" @default.
• W2023880109 crossrefType "journal-article" @default.
• W2023880109 hasAuthorship W2023880109A5048955712 @default.
• W2023880109 hasAuthorship W2023880109A5054810944 @default.
• W2023880109 hasAuthorship W2023880109A5057251750 @default.
• W2023880109 hasAuthorship W2023880109A5059952544 @default.
• W2023880109 hasAuthorship W2023880109A5066648363 @default.
• W2023880109 hasConcept C103408237 @default.
• W2023880109 hasConcept C104317684 @default.
• W2023880109 hasConcept C109129352 @default.
• W2023880109 hasConcept C143065580 @default.
• W2023880109 hasConcept C153911025 @default.
• W2023880109 hasConcept C16318435 @default.
• W2023880109 hasConcept C166342909 @default.
• W2023880109 hasConcept C181199279 @default.
• W2023880109 hasConcept C2775880066 @default.
• W2023880109 hasConcept C2776165026 @default.
• W2023880109 hasConcept C2776414213 @default.
• W2023880109 hasConcept C501734568 @default.
• W2023880109 hasConcept C515207424 @default.
• W2023880109 hasConcept C55493867 @default.
• W2023880109 hasConcept C75563809 @default.
• W2023880109 hasConcept C86803240 @default.
• W2023880109 hasConcept C96777560 @default.
• W2023880109 hasConceptScore W2023880109C103408237 @default.
• W2023880109 hasConceptScore W2023880109C104317684 @default.
• W2023880109 hasConceptScore W2023880109C109129352 @default.
• W2023880109 hasConceptScore W2023880109C143065580 @default.
• W2023880109 hasConceptScore W2023880109C153911025 @default.
• W2023880109 hasConceptScore W2023880109C16318435 @default.
• W2023880109 hasConceptScore W2023880109C166342909 @default.
• W2023880109 hasConceptScore W2023880109C181199279 @default.
• W2023880109 hasConceptScore W2023880109C2775880066 @default.
• W2023880109 hasConceptScore W2023880109C2776165026 @default.
• W2023880109 hasConceptScore W2023880109C2776414213 @default.
• W2023880109 hasConceptScore W2023880109C501734568 @default.
• W2023880109 hasConceptScore W2023880109C515207424 @default.
• W2023880109 hasConceptScore W2023880109C55493867 @default.
• W2023880109 hasConceptScore W2023880109C75563809 @default.
• W2023880109 hasConceptScore W2023880109C86803240 @default.
• W2023880109 hasConceptScore W2023880109C96777560 @default.
• W2023880109 hasIssue "1" @default.
• W2023880109 hasLocation W20238801091 @default.
• W2023880109 hasLocation W20238801092 @default.
• W2023880109 hasOpenAccess W2023880109 @default.
• W2023880109 hasPrimaryLocation W20238801091 @default.
• W2023880109 hasRelatedWork W1586318868 @default.
• W2023880109 hasRelatedWork W2007281810 @default.
• W2023880109 hasRelatedWork W2109034439 @default.
• W2023880109 hasRelatedWork W2147494701 @default.
• W2023880109 hasRelatedWork W2362481749 @default.
• W2023880109 hasRelatedWork W2409088546 @default.

• next