Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023885672> ?p ?o ?g. }
- W2023885672 endingPage "2072" @default.
- W2023885672 startingPage "2063" @default.
- W2023885672 abstract "Abstract Recombinant collagens are attractive proteins for a number of biomedical applications. To date, significant progress was made in the large‐scale production of nonmodified recombinant collagens; however, engineering of novel collagen‐like proteins according to customized specifications has not been addressed. Herein we investigated the possibility of rational engineering of collagen‐like proteins with specifically assigned characteristics. We have genetically engineered two DNA constructs encoding multi‐D4 collagens defined as collagen‐like proteins, consisting primarily of a tandem of the collagen II D4 periods that correspond to the biologically active region. We have also attempted to decrease enzymatic degradation of novel collagen by mutating a matrix metalloproteinase 1 cleavage site present in the D4 period. We demonstrated that the recombinant collagen α‐chains consisting predominantly of the D4 period but lacking most of the other D periods found in native collagen fold into a typical collagen triple helix, and the novel procollagens are correctly processed by procollagen N‐proteinase and procollagen C‐proteinase. The nonmutated multi‐D4 collagen had a normal melting point of 41°C and a similar carbohydrate content as that of control. In contrast, the mutant multi‐D4 collagen had a markedly lower thermostability of 36°C and a significantly higher carbohydrate content. Both collagens were cleaved at multiple sites by matrix metalloproteinase 1, but the rate of hydrolysis of the mutant multi‐D4 collagen was lower. These results provide a basis for the rational engineering of collagenous proteins and identifying any undesirable consequences of altering the collagenous amino acid sequences." @default.
- W2023885672 created "2016-06-24" @default.
- W2023885672 creator A5024633360 @default.
- W2023885672 creator A5062912778 @default.
- W2023885672 creator A5087778640 @default.
- W2023885672 creator A5088798957 @default.
- W2023885672 creator A5090529638 @default.
- W2023885672 date "2003-09-01" @default.
- W2023885672 modified "2023-09-30" @default.
- W2023885672 title "Prospects and limitations of the rational engineering of fibrillar collagens" @default.
- W2023885672 cites W1489869268 @default.
- W2023885672 cites W1539143178 @default.
- W2023885672 cites W1553932848 @default.
- W2023885672 cites W1580522854 @default.
- W2023885672 cites W1586161363 @default.
- W2023885672 cites W1588834418 @default.
- W2023885672 cites W190819802 @default.
- W2023885672 cites W1911305027 @default.
- W2023885672 cites W1940784716 @default.
- W2023885672 cites W1964556609 @default.
- W2023885672 cites W1965004892 @default.
- W2023885672 cites W1968288366 @default.
- W2023885672 cites W1968324625 @default.
- W2023885672 cites W1969179229 @default.
- W2023885672 cites W1974833130 @default.
- W2023885672 cites W1976772770 @default.
- W2023885672 cites W1985073091 @default.
- W2023885672 cites W1987512033 @default.
- W2023885672 cites W2008998538 @default.
- W2023885672 cites W2009436041 @default.
- W2023885672 cites W2011016569 @default.
- W2023885672 cites W2013443873 @default.
- W2023885672 cites W2015809567 @default.
- W2023885672 cites W2019365102 @default.
- W2023885672 cites W2021995652 @default.
- W2023885672 cites W2023519337 @default.
- W2023885672 cites W2024028932 @default.
- W2023885672 cites W2024943991 @default.
- W2023885672 cites W2025665629 @default.
- W2023885672 cites W2027521345 @default.
- W2023885672 cites W2027902728 @default.
- W2023885672 cites W2035040246 @default.
- W2023885672 cites W2036009126 @default.
- W2023885672 cites W2036354205 @default.
- W2023885672 cites W2037490596 @default.
- W2023885672 cites W2038997089 @default.
- W2023885672 cites W2049050052 @default.
- W2023885672 cites W2050060496 @default.
- W2023885672 cites W2052414130 @default.
- W2023885672 cites W2052577034 @default.
- W2023885672 cites W2052802440 @default.
- W2023885672 cites W2058547055 @default.
- W2023885672 cites W2082454321 @default.
- W2023885672 cites W2088418501 @default.
- W2023885672 cites W2097707959 @default.
- W2023885672 cites W2104449333 @default.
- W2023885672 cites W2111618967 @default.
- W2023885672 cites W2118762817 @default.
- W2023885672 cites W2129549504 @default.
- W2023885672 cites W2134924147 @default.
- W2023885672 cites W2140047620 @default.
- W2023885672 cites W2154387825 @default.
- W2023885672 cites W2155851139 @default.
- W2023885672 cites W2156564908 @default.
- W2023885672 cites W2171244584 @default.
- W2023885672 cites W2198115699 @default.
- W2023885672 cites W2395002117 @default.
- W2023885672 cites W2395882403 @default.
- W2023885672 cites W2413055077 @default.
- W2023885672 cites W255028057 @default.
- W2023885672 cites W2787992352 @default.
- W2023885672 cites W4254087246 @default.
- W2023885672 cites W4300941906 @default.
- W2023885672 doi "https://doi.org/10.1110/ps.0385103" @default.
- W2023885672 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2324002" @default.
- W2023885672 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12931004" @default.
- W2023885672 hasPublicationYear "2003" @default.
- W2023885672 type Work @default.
- W2023885672 sameAs 2023885672 @default.
- W2023885672 citedByCount "31" @default.
- W2023885672 countsByYear W20238856722012 @default.
- W2023885672 countsByYear W20238856722013 @default.
- W2023885672 countsByYear W20238856722014 @default.
- W2023885672 countsByYear W20238856722015 @default.
- W2023885672 countsByYear W20238856722017 @default.
- W2023885672 countsByYear W20238856722018 @default.
- W2023885672 countsByYear W20238856722020 @default.
- W2023885672 countsByYear W20238856722021 @default.
- W2023885672 countsByYear W20238856722022 @default.
- W2023885672 countsByYear W20238856722023 @default.
- W2023885672 crossrefType "journal-article" @default.
- W2023885672 hasAuthorship W2023885672A5024633360 @default.
- W2023885672 hasAuthorship W2023885672A5062912778 @default.
- W2023885672 hasAuthorship W2023885672A5087778640 @default.
- W2023885672 hasAuthorship W2023885672A5088798957 @default.
- W2023885672 hasAuthorship W2023885672A5090529638 @default.
- W2023885672 hasBestOaLocation W20238856722 @default.
- W2023885672 hasConcept C104317684 @default.