Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023887122> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2023887122 endingPage "69" @default.
- W2023887122 startingPage "64" @default.
- W2023887122 abstract "To determine the mutagenic and genotoxic properties of the major guanine N2-adduct formed by the antitumor drug mitomycin C, we have synthesized a decanucleotide, d(TTACG[MC]TATCT), containing the adduct, which was inserted into a gapped bacteriophage M13 genome. Analysis of the constructed genome indicated that 41% ligation of the adducted 10-mer occurred on both sides of the gap, whereas the control 10-mer ligated with 34% efficiency. After transfection of the adducted single-stranded M13 DNA into Escherichia coli, the adduct was found to be highly genotoxic. Viability of the adducted genome in a repair-competent strain was only 7%, which increased to 12% and 15% upon induction of SOS by irradiating the cells with 254-nm light at 20 and 50 J/m2, respectively. Even lower viability of 2%, 4.6%, and 0.2% was observed in uvrA, uvrB, and uvrC strains, respectively, which increased up to 10-fold with SOS. An examination of the surviving phage populations revealed that the adduct was not detectably mutagenic. No mutants from the repair-proficient strain were detected after analysis of more than 2500 progeny phage. Only 0.2% of the survivors were mutants in the uvrA strain. It is uncertain, however, if they were induced by the adduct, since all the mutants showed untargeted mutations. We conclude that the major guanine N2-adduct formed by mitomycin C is cytotoxic but not appreciably mutagenic in E. coli." @default.
- W2023887122 created "2016-06-24" @default.
- W2023887122 creator A5046503619 @default.
- W2023887122 creator A5049288091 @default.
- W2023887122 creator A5086726365 @default.
- W2023887122 creator A5065223818 @default.
- W2023887122 date "1998-01-01" @default.
- W2023887122 modified "2023-10-18" @default.
- W2023887122 title "The Major Mitomycin C−DNA Monoadduct Is Cytotoxic but Not Mutagenic in <i>Escherichia coli</i>" @default.
- W2023887122 cites W1552540582 @default.
- W2023887122 cites W18711077 @default.
- W2023887122 cites W1966264632 @default.
- W2023887122 cites W1985152732 @default.
- W2023887122 cites W2038478561 @default.
- W2023887122 cites W2049524424 @default.
- W2023887122 cites W2067646213 @default.
- W2023887122 cites W2075612078 @default.
- W2023887122 cites W2950392616 @default.
- W2023887122 cites W6051284 @default.
- W2023887122 doi "https://doi.org/10.1021/tx970163+" @default.
- W2023887122 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9477227" @default.
- W2023887122 hasPublicationYear "1998" @default.
- W2023887122 type Work @default.
- W2023887122 sameAs 2023887122 @default.
- W2023887122 citedByCount "21" @default.
- W2023887122 countsByYear W20238871222012 @default.
- W2023887122 countsByYear W20238871222014 @default.
- W2023887122 countsByYear W20238871222015 @default.
- W2023887122 countsByYear W20238871222016 @default.
- W2023887122 countsByYear W20238871222018 @default.
- W2023887122 countsByYear W20238871222019 @default.
- W2023887122 crossrefType "journal-article" @default.
- W2023887122 hasAuthorship W2023887122A5046503619 @default.
- W2023887122 hasAuthorship W2023887122A5049288091 @default.
- W2023887122 hasAuthorship W2023887122A5065223818 @default.
- W2023887122 hasAuthorship W2023887122A5086726365 @default.
- W2023887122 hasConcept C104317684 @default.
- W2023887122 hasConcept C105702510 @default.
- W2023887122 hasConcept C108204754 @default.
- W2023887122 hasConcept C136199206 @default.
- W2023887122 hasConcept C143065580 @default.
- W2023887122 hasConcept C153911025 @default.
- W2023887122 hasConcept C178790620 @default.
- W2023887122 hasConcept C185592680 @default.
- W2023887122 hasConcept C2776441376 @default.
- W2023887122 hasConcept C2776639595 @default.
- W2023887122 hasConcept C2778022156 @default.
- W2023887122 hasConcept C2778301229 @default.
- W2023887122 hasConcept C2779338040 @default.
- W2023887122 hasConcept C2781247443 @default.
- W2023887122 hasConcept C512185932 @default.
- W2023887122 hasConcept C54355233 @default.
- W2023887122 hasConcept C547475151 @default.
- W2023887122 hasConcept C552990157 @default.
- W2023887122 hasConcept C86803240 @default.
- W2023887122 hasConceptScore W2023887122C104317684 @default.
- W2023887122 hasConceptScore W2023887122C105702510 @default.
- W2023887122 hasConceptScore W2023887122C108204754 @default.
- W2023887122 hasConceptScore W2023887122C136199206 @default.
- W2023887122 hasConceptScore W2023887122C143065580 @default.
- W2023887122 hasConceptScore W2023887122C153911025 @default.
- W2023887122 hasConceptScore W2023887122C178790620 @default.
- W2023887122 hasConceptScore W2023887122C185592680 @default.
- W2023887122 hasConceptScore W2023887122C2776441376 @default.
- W2023887122 hasConceptScore W2023887122C2776639595 @default.
- W2023887122 hasConceptScore W2023887122C2778022156 @default.
- W2023887122 hasConceptScore W2023887122C2778301229 @default.
- W2023887122 hasConceptScore W2023887122C2779338040 @default.
- W2023887122 hasConceptScore W2023887122C2781247443 @default.
- W2023887122 hasConceptScore W2023887122C512185932 @default.
- W2023887122 hasConceptScore W2023887122C54355233 @default.
- W2023887122 hasConceptScore W2023887122C547475151 @default.
- W2023887122 hasConceptScore W2023887122C552990157 @default.
- W2023887122 hasConceptScore W2023887122C86803240 @default.
- W2023887122 hasIssue "1" @default.
- W2023887122 hasLocation W20238871221 @default.
- W2023887122 hasLocation W20238871222 @default.
- W2023887122 hasOpenAccess W2023887122 @default.
- W2023887122 hasPrimaryLocation W20238871221 @default.
- W2023887122 hasRelatedWork W1573835478 @default.
- W2023887122 hasRelatedWork W1966783360 @default.
- W2023887122 hasRelatedWork W1967835966 @default.
- W2023887122 hasRelatedWork W2007393960 @default.
- W2023887122 hasRelatedWork W2029343773 @default.
- W2023887122 hasRelatedWork W2079808186 @default.
- W2023887122 hasRelatedWork W2087927403 @default.
- W2023887122 hasRelatedWork W2133125857 @default.
- W2023887122 hasRelatedWork W2139700768 @default.
- W2023887122 hasRelatedWork W2403420157 @default.
- W2023887122 hasVolume "11" @default.
- W2023887122 isParatext "false" @default.
- W2023887122 isRetracted "false" @default.
- W2023887122 magId "2023887122" @default.
- W2023887122 workType "article" @default.