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- W2023890033 abstract "A soluble rat liver enzyme system was utilized to synthesize C14-labeled terpenol pyrophosphates from radioactive mevalonic acid. After heat inactivation of the animal enzymes the components of the incubation mixture served as substrate for the biosynthesis of phytoene by carrot or tomato plastids during an incubation of 16–18 hr. Incorporation of C14 into phytoene was proved through chromatography on alumina, catalytic hydrogenation and chromatography of the lycopersane on alumina, and gas chromatography followed by counting of trapped eluates for radioactivity. Experiments are reported which are consistent with the presumption that a C20 terpenol pyrophosphate (presumably geranyl geranyl pyrophosphate) served as the substrate for the synthesis of phytoene. Evidence is also presented that either TPN or DPN, and not the reduced nucleotides, served as the cofactor for this reaction in plastids and in more purified enzyme systems. The incorporation of C14 of terpenol pyrophosphates into carotenes other than phytoene was also achieved with tomato plastids. Proof for the incorporation of C14 into these compounds was obtained in the same way as for phytoene, except in the case of β-carotene and lycopene. These compounds were crystallized to constant specific radioactivity." @default.
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- W2023890033 date "1962-06-01" @default.
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- W2023890033 title "The biosynthesis of phytoene and other carotenes by enzymes of isolated higher plant plastids" @default.
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- W2023890033 doi "https://doi.org/10.1016/0003-9861(62)90115-7" @default.
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