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- W2023891646 abstract "We have constructed and characterised a glucose sensor using glucose oxidase (GOD) covalently attached to carboxylic acid polyethyleneglycol (PEG), called (PEG–GOD). This modified enzyme was entrapped afterwards within poly(3,4-ethylenedioxythiophene) (PEDT) films electrogenerated on glassy carbon (GC) electrodes. The composite (PEG–GOD/PEDT) film is more porous than the film without enzyme (PEDT+PEG). Data from electrochemical quartz microbalance (ECQM) and pH-stat experiments indicate a good relative activity of the modified enzyme, ca. 12–15%. Amperometric measurements, using ferrocenemethanol as the redox mediator, confirms that the modified enzyme is catalytically active. The effect of film thickness was also investigated. The sensitivities were quite similar for modified-GOD electrodes (ca. 3 mA cm−2 M−1) and unmodified-GOD electrodes (ca. 2.7 mA cm−2 M−1) but a better stability was obtained with modified PEG–GOD electrodes." @default.
- W2023891646 created "2016-06-24" @default.
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- W2023891646 date "2001-10-01" @default.
- W2023891646 modified "2023-10-01" @default.
- W2023891646 title "A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly(3,4-ethylenedioxythiophene) (PEDT) films" @default.
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- W2023891646 doi "https://doi.org/10.1016/s0022-0728(01)00595-2" @default.
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