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• W2023893552 abstract "A single-step multiplex RT-PCR was developed for the simultaneous and colourimetric detection of six RNA viruses (Cucumber mosaic virus, Cherry leaf roll virus, strawberry latent ringspot virus, Arabis mosaic virus, Olive latent-1 virus and Olive latent-2 virus) which infect olive trees. Six compatible primer set for one-step RT-PCR amplification in a single closed-tube and 3' digoxigenin labelled probes were designed in optimal, specific and conserved regions. The method has been assessed with 195 Spanish field olive trees, suggesting that approximately 1.5% of the tested material was infected by Cucumber mosaic virus and 0.5% by Cherry leaf roll virus. This method saves time and reagent costs compared with monospecific RT-PCR which needs several reactions for the same number of tests. Using colourimetric detection, it is possible to analyse many samples, it increases sensitivity 10-fold, and whilst facilitating the interpretation of results, it avoids the use of gels and the toxic ethidium bromide. The method could be used routinely for sanitary and certification programmes." @default.
• W2023893552 created "2016-06-24" @default.
• W2023893552 creator A5033137470 @default.
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• W2023893552 date "2001-07-01" @default.
• W2023893552 modified "2023-10-05" @default.
• W2023893552 title "Single-step multiplex RT-PCR for simultaneous and colourimetric detection of six RNA viruses in olive trees" @default.
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• W2023893552 doi "https://doi.org/10.1016/s0166-0934(01)00313-5" @default.
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• W2023893552 hasPublicationYear "2001" @default.
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