FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023922530> ?p ?o ?g. }

• W2023922530 endingPage "711" @default.
• W2023922530 startingPage "703" @default.
• W2023922530 abstract "Disodium aurothiomalate (AuTM) has been used successfully in the treatment of various autoimmune and inflammatory disorders; however, the molecular target(s) for this agent remains unknown. The aim of this study was to investigate whether the activity of CD45, a protein-tyrosine phosphatase (PTP, EC 3.1.3.48) essential for antigen-receptor-mediated lymphocyte signaling, was modified by AuTM exposure. The effects of AuTM on the activities of CD45 and other PTPs were monitored in vitro by a continuous assay using the substrate fluorescein diphosphate. In addition, the inhibition of PTP1B by AuTM was determined using a novel binding assay that employed an optical biosensor (BIAcore). The experimental results are summarized here: AuTM inhibited CD45 activity with an IC50 of 1.2 +/- 0.1 microM, and inhibition was competitive with substrate. The effect of AuTM, however, was not restricted to CD45, as the cytoplasmic PTP (PTP1B) was also inhibited, with an IC50 of 3.6 +/- 0.2 microM. AuTM also blocked the binding of GST-PTP1B to an immobilized active site inhibitor: a non-hydrolyzable difluorophosphonomethyl phenylalanine-containing biotinylated hexapeptide. AuTM-inhibited CD45 could be reactivated by the addition of excess dithiothreitol. These findings indicate that AuTM may interact with the essential active site cysteine residue involved in the catalytic mechanism of PTPs. Thus, it is possible that some of the cellular effects of gold result from the inhibition of these important cell signaling molecules." @default.
• W2023922530 created "2016-06-24" @default.
• W2023922530 creator A5042482808 @default.
• W2023922530 creator A5059226822 @default.
• W2023922530 creator A5061429410 @default.
• W2023922530 creator A5073724400 @default.
• W2023922530 date "1997-09-01" @default.
• W2023922530 modified "2023-10-13" @default.
• W2023922530 title "Mechanism of inhibition of protein-tyrosine phosphatases by disodium aurothiomalate" @default.
• W2023922530 cites W1501286282 @default.
• W2023922530 cites W1605580650 @default.
• W2023922530 cites W1965459549 @default.
• W2023922530 cites W1974867042 @default.
• W2023922530 cites W1990598353 @default.
• W2023922530 cites W1993145131 @default.
• W2023922530 cites W2015302394 @default.
• W2023922530 cites W2017582166 @default.
• W2023922530 cites W2019128910 @default.
• W2023922530 cites W2025485285 @default.
• W2023922530 cites W2026063837 @default.
• W2023922530 cites W2029080316 @default.
• W2023922530 cites W2032207545 @default.
• W2023922530 cites W2044529125 @default.
• W2023922530 cites W2050397685 @default.
• W2023922530 cites W2058148903 @default.
• W2023922530 cites W2066451762 @default.
• W2023922530 cites W2071548379 @default.
• W2023922530 cites W2075992939 @default.
• W2023922530 cites W2104594849 @default.
• W2023922530 cites W2113252814 @default.
• W2023922530 cites W2147078837 @default.
• W2023922530 cites W2165490555 @default.
• W2023922530 cites W2166002768 @default.
• W2023922530 cites W2181131337 @default.
• W2023922530 cites W2297407431 @default.
• W2023922530 cites W2951895863 @default.
• W2023922530 cites W4245747415 @default.
• W2023922530 doi "https://doi.org/10.1016/s0006-2952(97)00217-7" @default.
• W2023922530 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9310347" @default.
• W2023922530 hasPublicationYear "1997" @default.
• W2023922530 type Work @default.
• W2023922530 sameAs 2023922530 @default.
• W2023922530 citedByCount "31" @default.
• W2023922530 countsByYear W20239225302012 @default.
• W2023922530 countsByYear W20239225302013 @default.
• W2023922530 countsByYear W20239225302014 @default.
• W2023922530 countsByYear W20239225302015 @default.
• W2023922530 countsByYear W20239225302016 @default.
• W2023922530 countsByYear W20239225302018 @default.
• W2023922530 countsByYear W20239225302019 @default.
• W2023922530 countsByYear W20239225302020 @default.
• W2023922530 countsByYear W20239225302022 @default.
• W2023922530 crossrefType "journal-article" @default.
• W2023922530 hasAuthorship W2023922530A5042482808 @default.
• W2023922530 hasAuthorship W2023922530A5059226822 @default.
• W2023922530 hasAuthorship W2023922530A5061429410 @default.
• W2023922530 hasAuthorship W2023922530A5073724400 @default.
• W2023922530 hasConcept C107824862 @default.
• W2023922530 hasConcept C153911025 @default.
• W2023922530 hasConcept C170493617 @default.
• W2023922530 hasConcept C178666793 @default.
• W2023922530 hasConcept C181199279 @default.
• W2023922530 hasConcept C185592680 @default.
• W2023922530 hasConcept C202751555 @default.
• W2023922530 hasConcept C204628709 @default.
• W2023922530 hasConcept C2777752497 @default.
• W2023922530 hasConcept C2777824588 @default.
• W2023922530 hasConcept C2779201268 @default.
• W2023922530 hasConcept C41183919 @default.
• W2023922530 hasConcept C55493867 @default.
• W2023922530 hasConcept C85528070 @default.
• W2023922530 hasConcept C86803240 @default.
• W2023922530 hasConceptScore W2023922530C107824862 @default.
• W2023922530 hasConceptScore W2023922530C153911025 @default.
• W2023922530 hasConceptScore W2023922530C170493617 @default.
• W2023922530 hasConceptScore W2023922530C178666793 @default.
• W2023922530 hasConceptScore W2023922530C181199279 @default.
• W2023922530 hasConceptScore W2023922530C185592680 @default.
• W2023922530 hasConceptScore W2023922530C202751555 @default.
• W2023922530 hasConceptScore W2023922530C204628709 @default.
• W2023922530 hasConceptScore W2023922530C2777752497 @default.
• W2023922530 hasConceptScore W2023922530C2777824588 @default.
• W2023922530 hasConceptScore W2023922530C2779201268 @default.
• W2023922530 hasConceptScore W2023922530C41183919 @default.
• W2023922530 hasConceptScore W2023922530C55493867 @default.
• W2023922530 hasConceptScore W2023922530C85528070 @default.
• W2023922530 hasConceptScore W2023922530C86803240 @default.
• W2023922530 hasIssue "6" @default.
• W2023922530 hasLocation W20239225301 @default.
• W2023922530 hasLocation W20239225302 @default.
• W2023922530 hasOpenAccess W2023922530 @default.
• W2023922530 hasPrimaryLocation W20239225301 @default.
• W2023922530 hasRelatedWork W1965374807 @default.
• W2023922530 hasRelatedWork W1968905431 @default.
• W2023922530 hasRelatedWork W1976873115 @default.
• W2023922530 hasRelatedWork W2017595667 @default.
• W2023922530 hasRelatedWork W2049117872 @default.
• W2023922530 hasRelatedWork W2057291091 @default.

• next