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• W2023931058 abstract "The possible role of homodimerization events in intracellular signal transduction triggered by the bipartite human interleukin-4 receptor was addressed. We generated cell lines functionally expressing derivatives of the two receptor subunits α and γ, which allow for a specific and background-free experimental induction of intracellular homo- and heterodimers. A heterodimer of α and γ released an intracellular signal, whereas a γ-γ homodimer did not. Unexpectedly, we found the intracellular domain of interleukin-4 receptor α chain to evoke cell proliferation and activation of tyrosine kinase Jak1 as well as of transcription factor Stat6 upon homodimerization. Both recruitment of the common γ chain and activation of kinase Jak3 were shown to be dispensible for these processes. The possible role of homodimerization events in intracellular signal transduction triggered by the bipartite human interleukin-4 receptor was addressed. We generated cell lines functionally expressing derivatives of the two receptor subunits α and γ, which allow for a specific and background-free experimental induction of intracellular homo- and heterodimers. A heterodimer of α and γ released an intracellular signal, whereas a γ-γ homodimer did not. Unexpectedly, we found the intracellular domain of interleukin-4 receptor α chain to evoke cell proliferation and activation of tyrosine kinase Jak1 as well as of transcription factor Stat6 upon homodimerization. Both recruitment of the common γ chain and activation of kinase Jak3 were shown to be dispensible for these processes. Interleukin-4 (IL-4) 1The abbreviations used are: ILinterleukinhIL-4human interleukin-4hIL-4Rhuman interleukin-4 receptorhIL-4Rαhuman interleukin-4 receptor α chainγccommon receptor γ chainJakjanus kinaseStatsignal transducer and activator of transcriptionα-anti-. is a pleiotropic immune regulator with a pivotal role in certain allergic processes (1Paul W.E. Blood. 1991; 77: 1859-1870Google Scholar). The bipartite IL-4 receptor comprises the interleukin-4 receptor α chain (IL-4Rα) (2Idzerda R.L. March C.J. Mosley B. Lyman S.D. Bos T.V. Gimpel S.D. Din W.S. Grabstein K.H. Widmer M.B. Park L.S. Cosman D. Beckman M.P. J. Exp. Med. 1990; 171: 861-973Google Scholar) and the common γ receptor chain (γc) (3Russell S.M. Keegan A.D. Harada N. Nakamura Y. Noguchi M. Leland P. Friedman M.C. Miyajima A. Puri R.K. Paul W.E. Leonard W.J. Science. 1993; 262: 1880-1883Google Scholar, 4Kondo M. Takeshita T. Ishii N. Nakamura M. Watanabe S. Arai K. Sugamura K. Science. 1993; 262: 1874-1879Google Scholar). Both receptor subunits belong to the cytokine receptor superfamily (5Bazan J.F. Immunol. Today. 1990; 11: 350-354Google Scholar) and are shared by other cytokines; γc is also part of the receptors for IL-2, IL-7, IL-9, and IL-15 (6Sugamura K. Asao H. Kondo M. Tanaka N. Ishii N. Nakamura M. Takeshita T. Adv. Immunol. 1995; 59: 225-277Google Scholar), and IL-4Rα contributes to the IL-13 receptor (7Smerz-Bertling C. Duschl A. J. Biol. Chem. 1995; 270: 966-970Google Scholar, 8Obiri N.I. Debinski W. Leonard W.J. Puri R.K. J. Biol. Chem. 1995; 270: 8797-8804Google Scholar). interleukin human interleukin-4 human interleukin-4 receptor human interleukin-4 receptor α chain common receptor γ chain janus kinase signal transducer and activator of transcription anti-. Ligand-induced juxtaposition of the cytoplasmic domains of IL-4Rα and γc is believed to be a mandatory step in intracellular signaling which involves recruitment and activation of kinases Jak1 and Jak3 (9Yin T. Tsang M.L.-S. Yang Y.-C. J. Biol. Chem. 1994; 269: 26614-26617Google Scholar, 10Witthuhn B.A. Silvennoinen O. Miura O. Lai K.S. Cwik C. Liu E.T. Ihle J.N. Nature. 1994; 370: 153-157Google Scholar), transcription factor Stat6 (11Hou J. Schindler U. Henzel W.J. Ho T.C. Brasseur M. McKnight S.L. Science. 1994; 265: 1701-1706Google Scholar), and the adaptor molecule IRS-2 (12Keegan A.D. Nelms K. White M. Wang L.-M. Pierce J.H. Paul W.E. Cell. 1994; 76: 811-820Google Scholar). However, the architecture of the IL-4R complex as well as the molecular mechanisms underlying the specificity of IL-4-induced signal transduction are to date poorly understood. Making use of the strictly species-specific interaction of interleukin-4 with IL-4Rα chain, factor-dependent murine cells were rendered responsive to hIL-4 by expressing human IL-4Rα (2Idzerda R.L. March C.J. Mosley B. Lyman S.D. Bos T.V. Gimpel S.D. Din W.S. Grabstein K.H. Widmer M.B. Park L.S. Cosman D. Beckman M.P. J. Exp. Med. 1990; 171: 861-973Google Scholar, 13Harada N. Yang G. Miyajima A. Howard M. J. Biol. Chem. 1992; 267: 22752-22758Google Scholar, 14Seldin D.C. Leder P. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 2140-2144Google Scholar, 15Koettnitz K. Kalthoff F.S. Eur. J. Immunol. 1993; 23: 988-991Google Scholar, 16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar). An implication of these results is the ability of human IL-4 to activate IL-4 receptor complexes containing either human or murine common γ chain, thus complicating an analysis of the composition of the signaling competent receptor subunit assembly. In order to study the role of receptor chain dimerization events in signal release by the hIL-4R complex, we generated an expression system for receptor subunits that allowed us to experimentally induce specific and background-free intracellular hetero- and homodimerization. Our results show that the juxtaposition of two intracellular domains of IL-4Rα can act as the trigger of specific signaling, including the activation of Jak1 and Stat6 and the induction of cell proliferation. Surprisingly, a hitherto assumed participation of the cytoplasmic portion of common γ chain and of γc-associated kinase Jak3 is not required. Recombinant DNA work was performed according to standard procedures (17Sambrook J. Fritsch E.F. Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY1989Google Scholar). The murine pre-B cell line Ba/F3 (18Palacios R. Steinmetz M. Cell. 1985; 41: 727-734Google Scholar) has been described. BAF-4α-pγ, a Ba/F3 derivative expressing both subunits of the human IL-4R, is identical to BAF-4Rhγ (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar). Hybrid receptor genes were generated by polymerase chain reaction amplification of gene fragments from pKCR-pγ (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar) encoding the epitope-tagged extracellular domain and transmembrane/intracellular domain of human γc and exchanging them for the corresponding fragments (BamHI/XhoI or XhoI/HindIII) in pKCR-4α. The resulting expression plasmids pKCR-4α/γ and pKCR-pγ/α were cotransfected into Ba/F3 cells as described (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar). Surface expression of receptor constructs was assayed by reacting intact cells with antibodies X 14/38 (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar, 19Bönsch D. Kammer W. Lischke A. Friedrich K. J. Biol. Chem. 1995; 270: 8452-8457Google Scholar) or P5D4 (20Kreis T.E. Lodish H.F. Cell. 1986; 46: 929-937Google Scholar) specific for the extracellular portions of recombinant hIL-4Rα or epitope-tagged human γc, respectively, and subsequent detection of bound antibodies by peroxidase-coupled secondary antibodies as detailed elsewhere (21Lischke A. Pagany M. Kammer W. Friedrich K. Anal. Biochem. 1996; 236: 322-326Google Scholar). Briefly, 105 cells in a microtiter well were incubated on ice for 30 min with 5 μg of antibody in a volume of 50 μl of phosphate-buffered saline/3% bovine serum albumin. After washing twice, cells were resuspended in 100 μl of a 100 μg/ml solution of peroxidase-conjugated goat anti-mouse IgG (Dianova) and kept on ice for 30 min. Cell-bound secondary antibody was detected by transferring the cells to 50 μl of a solution containing 0.1 M Tris/HCl, pH 8.5, 2.5 mM 3-aminophthalhydrazide (Fluka), 400 μM p-coumaric acid (Sigma), 5.4 mM H2O2 and measuring elicited chemiluminescence using a MicroLumat LB 96P. Quantitation of surface-bound antibody molecules was achieved by relating the determined intensity of luminescence to a calibration series of samples containing known concentrations of peroxidase. Cell maintainance and preparation of hIL-4 and mutant Y124D has been described previously (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar). Recombinant murine IL-4 was purchased from Sigma. Cytokine-induced proliferation of cell lines was measured by [3H] thymidine incorporation into de novo synthesized DNA as described (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar). Samples of 3 × 107 cells were incubated at 37°C for 10 min in 1 ml of RPMI containing no cytokine, 7 nM of IL-4, or 50 nM of antibody P5D4 and subsequently lysed as described (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar). Cleared lysates were incubated with 1-5 μg of specific antibody. Antibodies used for immunoprecipitations were 4G10 (anti-phosphotyrosine, Upstate Biotechnology), E34-1 (22Gurniak C.B. Berg L.J. Blood. 1996; 87: 3151-3160Google Scholar) and anti-Jak1 rabbit serum (23Wilks A.F. Harpur A.G. Kurban R.R. Ralph S.J. Zürcher G. Ziemiecki A. Mol. Cell. Biol. 1991; 11: 2057-2065Google Scholar). Immunocomplexes were precipitated from lysates with 50 μl of anti-mouse IgG-agarose or protein A-Sepharose (Sigma) and assayed as described (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar) using peroxidase-conjugated antibody RC20 (Transduction Laboratories) at a final concentration of 0.1 μg/ml. Iodination of hIL-4, cross-linking of radioligand to cell-surface receptors, and analysis of immunoprecipitated complexes by electrophoresis was carried out as described (19Bönsch D. Kammer W. Lischke A. Friedrich K. J. Biol. Chem. 1995; 270: 8452-8457Google Scholar). Whole cell extracts were prepared from cells stimulated with IL-4 or antibody as described above by suspension of cell pellets in a buffer containing 20 mM Hepes, pH 7.9, 400 mM NaCl, 1 mM EDTA, 20% glycerol, 1 mM dithiothreitol, 0.2 mM phenylmethylsulfonyl fluoride, 5 μg/ml leupeptin, 5 μg/ml, and 100 μM sodium ortho-vanadate followed by three freeze-thaw cycles and centrifugation at 4°C and 14,000 rpm for 15 min. Supernatants equivalent to 105 cells were used for bandshift assays performed as described (24Wakao H. Schmitt-Ney Groner B. J. Biol. Chem. 1992; 267: 16365-16370Google Scholar). As a probe, the Stat6-binding sequence 5′-GTCAACTTCCCAAGAACAGAA-3′ derived from the human Iϵ-promoter (25Köhler I. Rieber E.P. Eur. J. Immunol. 1993; 23: 3066-3071Google Scholar) end-labeled with polynucleotide kinase to a specific activity of 8.000 cpm/fmol was applied. Supershifting of Stat6 containing complexes was achieved by adding to the binding reactions before electrophoretic mobility shift assay 1 μg of a chicken antibody directed to amino acids 637-847 of murine Stat6. 2M. Heim and R. Moriggl, unpublished data. We intended to reconstitute in murine cells a functional interleukin-4 receptor complex activable exclusively by human IL-4, which would not evoke any background signaling due to interference with the endogenous murine IL-4 receptor. To this end, we generated a pair of expression constructs encoding hybrid receptor chains derived from hIL-4Rα and hγc with mutually exchanged intracellular domains (Fig. 1A) and introduced it into the murine pre-B cell line Ba/F3. One clone expressing both 4α/γ and pγ/4α chimeras was termed BAF-4α/γ-pγ/4α. The number of surface-expressed receptor molecules per cell was determined in comparison with cell line BAF-4α-pγ bearing both subunits of the authentic human IL-4R (Fig. 1B). As measured by the binding of specific antibodies recognizing the extracellular receptor domains, in both cell lines surface expression of the receptor chain comprising the intracellular domain of γc was considerably higher than that of the subunit bearing the intracellular part of hIL-4Rα. Irrespective of the “authentic” or “cross-over” composition of the heterologous subunits, similar hIL-4 binding receptor complexes could be formed in both cell lines as revealed by immunoprecipitation of receptor chains cross-linked to radiolabeled hIL-4 (Fig. 1C). To test if the bipartite human IL-4R with exchanged cytoplasmic domains was capable of transmitting specific signals to the cell interior, we measured IL-4-induced cell proliferation. When stimulated with hIL-4, BAF-4α/γ-pγ/4α cells expressing the combination of hybrid receptors, like BAF-4α-pγ cells, showed a proliferative response (Fig. 2A). In BAF-4α-pγ cells, hIL-4 mutant Y124D evoked 60% of the DNA synthesis induced by wild type IL-4. We have previously shown that this degree of reactivity is due to preferential interaction of Y124D with murine γc (16Lischke A. Kammer W. Friedrich K. Eur. J. Biochem. 1995; 234: 100-107Google Scholar). When assaying BAF-4α/γ-pγ/4α cells, we found, as earlier observed with human IL-4 reactive cells (19Bönsch D. Kammer W. Lischke A. Friedrich K. J. Biol. Chem. 1995; 270: 8452-8457Google Scholar), only 30% of wild type activity for hIL-4 variant Y124D. This result indicates that hIL-4 cross-over receptor, as anticipated and unlike its authentic counterpart, precludes the formation of productive receptor complexes involving endogenous murine common γ chain. Stimulation with hIL-4 resulted in equivalent patterns of tyrosine-phosphorylated proteins in the two cell lines (Fig. 2B). The intracellular domain of hIL-4Rα is a major substrate of ligand-induced phosphorylation as revealed by specific immunoprecipitation (data not shown). Moreover, the modified hIL-4 receptor was found to recapitulate hIL-4-specific activation of janus kinases Jak1 and Jak3. We next employed the model receptor system to address the individual roles of the IL-4 receptor subunits in signaling. The ligand and antibody binding properties of the functionally expressed receptor constructs enabled us to specifically induce all three possible intracellular receptor dimers (Fig. 3A). In BAF-4α/γ-pγ/4α cells, not only hIL-4-induced heterodimerization of the two intracellular receptor domains but surprisingly also antibody-mediated cytoplasmic homodimerization of hIL-4Rα via the extracellular P5D4 epitope tag lead to cell proliferation (Fig. 3B). Antibody-induced homodimerization of γc intracellular domains in BAF-4α-pγ cells did not result in elevated DNA synthesis. The concentrations of hIL-4 and antibody P5D4, respectively, eliciting a proliferative response are in concordance with reported dissociation constants for the binding of hIL-4 to the high affinity hIL-4R of (100 pM) (26Kruse N. Shen B.-J. Arnold S. Tony H.-P. Müller T. Sebald W. EMBO J. 1993; 13: 5121-5129Google Scholar) and for the interaction between antibody P5D4 and its cognate epitope (100 nM) (21Lischke A. Pagany M. Kammer W. Friedrich K. Anal. Biochem. 1996; 236: 322-326Google Scholar). The bell-shaped dose-response curve for the antibody-activity on BAF-4α/γ-pγ/4α cells indicates a blocking of receptor cross-linking by monovalent antibody binding at excess concentration and thus underscores our notion of P5D4-induced receptor homodimerization causing proliferation. Comparing the activation of janus kinases known to be involved in IL-4 receptor complex function by hetero- or homodimerization, respectively (Fig. 3C), we found that antibody-induced intracellular homodimerization of hIL-4Rα results in tyrosine phosphorylation of Jak1 but not of Jak3. Homodimerization of intracellular γc does not lead to a detectable phosphorylation of Jak1 or Jak3, whereas the heterodimer of α and γ evokes the activation of both kinases. Activation of Stat6, as assayed by its property to bind to a cognate DNA sequence derived from the Iϵ-promoter, is induced not only by an intracellular heterodimer of α and γ but also by an α-α homodimer (Fig. 3D). From these results we conclude that both the cytoplasmic domain of γc and activated Jak3 are not mandatory for Stat activation and for the onset of a signaling cascade leading to cell proliferation. The essential trigger for the release of these events is rather the juxtaposition of two intracellular domains of IL-4 receptor α chain and the concomitant activation of Jak1 by tyrosine phosphorylation. It is to date poorly understood how the common γ chain contributes to signaling mediated by different cytokine receptor complexes and how specificity of these receptors is achieved despite their sharing of a subunit. The only defined biochemical function of γc is the recruitment of Jak3 to the receptor complex (27Nelson B.H. Lord J.D. Greenberg P.D. Mol. Cell. Biol. 1996; 16: 309-317Google Scholar). This very process, however, has been shown not to be essential for the specific activity of the IL-2 receptor; it can rather be replaced by artificially introducing Jak2 into the assembly (28Lai S.Y. Xu W. Gaffen S.L. Liu K.D. Longmore G.D. Greene W.C. Goldsmith M.A. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 231-235Google Scholar). In this report we show that in the human IL-4 receptor system neither γc and Jak3 nor any substitute is required for the release of an intracellular signal if the intracellular domain of hIL-4Rα is experimentally homodimerized. Our results raise new questions about the role of γc in the function of the IL-4 receptor. The interaction of ligand with the extracellular domains of both IL-4Rα and γc is necessary for IL-4-induced signal transduction, because mutant forms of hIL-4 defective in contacting γc fail to stimulate cell proliferation (26Kruse N. Shen B.-J. Arnold S. Tony H.-P. Müller T. Sebald W. EMBO J. 1993; 13: 5121-5129Google Scholar, 29Duschl A. Eur. J. Biochem. 1995; 228: 305-310Google Scholar). Functional properties of the intracellular domain of γc in the activation of this particular receptor system have not yet been addressed. Our data indicate that it is not involved in the release of intracellular signals specific for IL-4 and support the notion of a more general role for γc in the formation of the signaling competent IL-4R and probably also other cytokine receptor complexes. In ligand-induced IL-4R activation, one function of γc and Jak3 could be the promotion of a transient assembly of two or more copies of hIL-4Rα, a situation which in turn would lead to specific intracellular signal transduction. Alternatively, in the natural receptor complex, γc-mediated recruitment of Jak3 might result in an activation of Jak1, an event that in our model experiment is mimicked by the juxtaposition of two Jak1 molecules and serves as the master trigger for the various activities of hIL-4Rα. A more general version of such an interpretation of exchangeable Jaks in the hIL-4R complex would be the view that ligand-induced intracellular apposition of several combinations of two Jak molecules would suffice to evoke cell proliferation and the other reactions observed. In this scenario, the major function of the specific receptor chain (here: hIL-4Rα) would be to provide recognition sites for Stats and other downstream components that upon Jak-driven activation mediate the particular effects of IL-4. Directed homodimerization of γc does not result in similiar activities because of its lack of recognition sites for downstream signaling molecules. Also in line with such an explanation would be the notion of cytokine receptor signal transduction being relatively unselective and flexible in terms of interactions between receptor chains and intracellular binding partners. This would imply that the main event regulating specificity in cytokine signaling is the recognition between receptor and ligand and the thereby cross-linked combination of receptor subunits. To discriminate between the two principal explanations compatible with our results (involvement of receptor multimers in “natural” hIL-4 receptor activation or low specificity of Jak activity combined with recruitment of signaling molecules by hIL-4Rα via specific recognition sites), careful investigation of the stoichiometric subunit composition of the active hIL-4 receptor complex and a mutational analysis of the cytoplasmic portion of γc in this context are necessary. Also, the molecular details of Jak recognition, activation, and specificity in the hIL-4R assembly have to be addressed. The expert technical assistance of C. Müller is gratefully acknowledged. We thank W. Sebald for generous support and cytokines and T. Kreis for antibody P5D4." @default.
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