FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023936385> ?p ?o ?g. }

• W2023936385 endingPage "2301" @default.
• W2023936385 startingPage "2292" @default.
• W2023936385 abstract "The fd bacteriophage is a filamentous virus consisting of a circular single-stranded DNA (ssDNA) wrapped by thousands of copies of a major coat protein subunit (the capsid). The coat protein subunits are mostly α-helical and curved, and are arranged in the capsid in consecutive pentamers related by a translation along the main viral axis and a rotation of ∼36° (C5S2 symmetry). The DNA is right-handed and helical, but information on its structure and on its interface with the capsid is incomplete. We present here an approach for assigning the DNA nucleotides and studying its interactions with the capsid by magic-angle spinning solid-state NMR. Capsid contacts with the ssDNA are obtained using a two-dimensional 13C–13C correlation experiment and a proton-mediated 31P–13C polarization transfer experiment, both acquired on an aromatic-unlabeled phage sample. Our results allow us to map the residues that face the interior of the capsid and to show that the ssDNA–capsid interactions are sustained mainly by electrostatic interactions between the positively charged lysine side chains and the phosphate backbone. The use of natural abundance aromatic amino acids in the growth media facilitated the complete assignment of the four nucleotides and the observation of internucleotide contacts. Using chemical shift analysis, our study shows that structural features of the deoxyribose carbons reporting on the sugar pucker are strikingly similar to those observed recently for the Pf1 phage. However, the ssDNA–protein interface is different, and chemical shift markers of base pairing are different. This experimental approach can be utilized in other filamentous and icosahedral bacteriophages, and also in other biomolecular complexes involving structurally and functionally important DNA–protein interactions." @default.
• W2023936385 created "2016-06-24" @default.
• W2023936385 creator A5013886780 @default.
• W2023936385 creator A5021683829 @default.
• W2023936385 creator A5048925276 @default.
• W2023936385 date "2014-01-30" @default.
• W2023936385 modified "2023-09-27" @default.
• W2023936385 title "Complete Chemical Shift Assignment of the ssDNA in the Filamentous Bacteriophage fd Reports on Its Conformation and on Its Interface with the Capsid Shell" @default.
• W2023936385 cites W1508259544 @default.
• W2023936385 cites W1966420704 @default.
• W2023936385 cites W1966438745 @default.
• W2023936385 cites W1968189773 @default.
• W2023936385 cites W1970225178 @default.
• W2023936385 cites W1971041971 @default.
• W2023936385 cites W1975483279 @default.
• W2023936385 cites W1976666996 @default.
• W2023936385 cites W1977416340 @default.
• W2023936385 cites W1983410419 @default.
• W2023936385 cites W1985109525 @default.
• W2023936385 cites W1986442327 @default.
• W2023936385 cites W1987014622 @default.
• W2023936385 cites W1987580271 @default.
• W2023936385 cites W1993823146 @default.
• W2023936385 cites W1999601914 @default.
• W2023936385 cites W2002062489 @default.
• W2023936385 cites W2002395437 @default.
• W2023936385 cites W2002869713 @default.
• W2023936385 cites W2004360966 @default.
• W2023936385 cites W2004764094 @default.
• W2023936385 cites W2008913102 @default.
• W2023936385 cites W2010099161 @default.
• W2023936385 cites W2016710603 @default.
• W2023936385 cites W2017751881 @default.
• W2023936385 cites W2017913827 @default.
• W2023936385 cites W2020421738 @default.
• W2023936385 cites W2022483898 @default.
• W2023936385 cites W2022943234 @default.
• W2023936385 cites W2023596283 @default.
• W2023936385 cites W2025799912 @default.
• W2023936385 cites W2027569419 @default.
• W2023936385 cites W2029486358 @default.
• W2023936385 cites W2033781578 @default.
• W2023936385 cites W2034121963 @default.
• W2023936385 cites W2035628504 @default.
• W2023936385 cites W2036921365 @default.
• W2023936385 cites W2038399334 @default.
• W2023936385 cites W2038853976 @default.
• W2023936385 cites W2044918102 @default.
• W2023936385 cites W2046353808 @default.
• W2023936385 cites W2051787622 @default.
• W2023936385 cites W2054802640 @default.
• W2023936385 cites W2056069359 @default.
• W2023936385 cites W2056526353 @default.
• W2023936385 cites W2058293114 @default.
• W2023936385 cites W2061627498 @default.
• W2023936385 cites W2062711694 @default.
• W2023936385 cites W2063216794 @default.
• W2023936385 cites W2065453923 @default.
• W2023936385 cites W2067520392 @default.
• W2023936385 cites W2068363397 @default.
• W2023936385 cites W2069258864 @default.
• W2023936385 cites W2075450229 @default.
• W2023936385 cites W2078043522 @default.
• W2023936385 cites W2079137593 @default.
• W2023936385 cites W2081279009 @default.
• W2023936385 cites W2085570760 @default.
• W2023936385 cites W2086496769 @default.
• W2023936385 cites W2086565576 @default.
• W2023936385 cites W2088629679 @default.
• W2023936385 cites W2089227855 @default.
• W2023936385 cites W2089300962 @default.
• W2023936385 cites W2089464030 @default.
• W2023936385 cites W2093535760 @default.
• W2023936385 cites W2093700493 @default.
• W2023936385 cites W2098942968 @default.
• W2023936385 cites W2099039280 @default.
• W2023936385 cites W2099182007 @default.
• W2023936385 cites W2107746113 @default.
• W2023936385 cites W2110001654 @default.
• W2023936385 cites W2117108962 @default.
• W2023936385 cites W2118250336 @default.
• W2023936385 cites W2131537621 @default.
• W2023936385 cites W2136460606 @default.
• W2023936385 cites W2138619586 @default.
• W2023936385 cites W2139780469 @default.
• W2023936385 cites W2142951496 @default.
• W2023936385 cites W2156078935 @default.
• W2023936385 cites W2156813690 @default.
• W2023936385 cites W2164113581 @default.
• W2023936385 cites W2165805570 @default.
• W2023936385 cites W2169821755 @default.
• W2023936385 cites W2314796390 @default.
• W2023936385 cites W2324808361 @default.
• W2023936385 cites W2327032117 @default.
• W2023936385 cites W2409941786 @default.
• W2023936385 cites W4205349198 @default.
• W2023936385 cites W4229743141 @default.
• W2023936385 doi "https://doi.org/10.1021/ja412178n" @default.

• next