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- W2023951242 abstract "The application of the fluorescent intercalating dye, YO-PRO-1 to the analysis of polymerase chain reaction-generated DNA fragments from 120 to 400 base pairs in size was investigated. Analysis of samples was performed using a non-gel sieving buffer containing Tris—borate, hydroxyethyl cellulose, and the intercalating dye. Minimum detectable concentrations were less than 500 pg/ml DNA. For samples requiring resolution of four base pairs or less, it was necessary to add a second intercalating agent, ethidium bromide to the buffer. Using this procedure, a number of loci of interest in genetic typing were examined." @default.
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- W2023951242 date "1993-10-01" @default.
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- W2023951242 title "Capillary electrophoresis of polymerase chain reaction-amplified DNA using fluorescence detection with an intercalating dye" @default.
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- W2023951242 doi "https://doi.org/10.1016/0021-9673(93)80647-q" @default.
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