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- W2023954274 abstract "The distribution of PAM was studied immunohistochemically at light and electron microscopic levels in the endocrine organs of mature male rats. PAM was found in the anterior pituitary gland, thyroid C cells, the gastrointestinal tract, the pancreas, and the adrenal medulla. For light microscopic immunohistochemistry, fixative B (pH 4 Bouin's) and paraffin embedding were satisfactory with SDS treatment preceding immunohistochemical staining. But PAM in the thyroid C cells could be detected only by frozen sections after Zamboni fixation. By the immunoelectron microscopy pre-embedding method, PAM was localized in secretory granules suggesting the site of a-amidation in secretory granules. Our studies emphasized the role of the secretory granules in posttranslational processing not only in proteolytic digestion but also in α-amidation." @default.
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- W2023954274 date "1993-01-01" @default.
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- W2023954274 title "Light and Electron Microscopic Immunohistochemical Localization of Peptidylglycine Alpha-Amidating Monooxygenase (PAM) in the Rat Endocrine Organs." @default.
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- W2023954274 doi "https://doi.org/10.1267/ahc.26.179" @default.
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