FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023954727> ?p ?o ?g. }

• W2023954727 endingPage "109" @default.
• W2023954727 startingPage "102" @default.
• W2023954727 abstract "Horseradish peroxidase isozyme C1a (HRP C1a) is widely used as a reporter enzyme in a variety of detection procedures such as enzyme-linked immunosorbent assay (ELISA) and western blotting. We previously isolated the gene encoding HRP C1a and showed that HRP C1a is at first translated as a preproprotein containing propeptides at its N- and C-termini (N-terminal secretion signal peptide and C-terminal propeptide; CTPP). The signal peptide (sp) is necessary for endoplasmic reticulum (ER) translocation and the CTPP acts as a vacuolar sorting determinant. Furthermore, HRP C1a was secreted into the culture medium from cells expressing the HRP C1a gene without the CTPP region. We optimized the secretory production system of HRP C1a in tobacco plants. To determine a suitable signal peptide for high-efficient secretion of proteins, three types of sp derived from HRP C1a (C1Psp), β- d -glucan exohydrolase (GEsp) and 38 kDa peroxidase (38Psp) were compared. GE and 38P are secretory proteins highly accumulated in the culture medium of BY2 cells. The secretion efficiency was increased by 34% and 53% when GEsp and 38Psp was used, respectively. Next, we used a translational enhancer, the 5′-untranslated region of Nicotiana tabacum alcohol dehydrogenase gene ( NtADH 5′-UTR). The production of HRP C1a was increased by placing NtADH 5′UTR in front of the ORF in BY2 cells. These results indicate that the localization and expression level of recombinant proteins can be controlled by the use of propeptides and 5′UTR, respectively. Finally, high-efficiency secretory production of the HRP C1a was also achieved in transgenic tobacco." @default.
• W2023954727 created "2016-06-24" @default.
• W2023954727 creator A5002406091 @default.
• W2023954727 creator A5023419492 @default.
• W2023954727 creator A5025070827 @default.
• W2023954727 creator A5034075544 @default.
• W2023954727 creator A5079582179 @default.
• W2023954727 creator A5090298115 @default.
• W2023954727 date "2006-08-01" @default.
• W2023954727 modified "2023-09-25" @default.
• W2023954727 title "High-efficiency secretory production of peroxidase C1a using vesicular transport engineering in transgenic tobacco" @default.
• W2023954727 cites W1591151754 @default.
• W2023954727 cites W1650320312 @default.
• W2023954727 cites W1767015811 @default.
• W2023954727 cites W1954713366 @default.
• W2023954727 cites W1982337997 @default.
• W2023954727 cites W1989002370 @default.
• W2023954727 cites W1993771217 @default.
• W2023954727 cites W1995371719 @default.
• W2023954727 cites W2003759516 @default.
• W2023954727 cites W2005758343 @default.
• W2023954727 cites W2006822851 @default.
• W2023954727 cites W2009651610 @default.
• W2023954727 cites W2011239271 @default.
• W2023954727 cites W2021488880 @default.
• W2023954727 cites W2027468656 @default.
• W2023954727 cites W2028062152 @default.
• W2023954727 cites W2030246735 @default.
• W2023954727 cites W2039971100 @default.
• W2023954727 cites W2046620625 @default.
• W2023954727 cites W2050868630 @default.
• W2023954727 cites W2057520156 @default.
• W2023954727 cites W2061813158 @default.
• W2023954727 cites W2063328591 @default.
• W2023954727 cites W2069078351 @default.
• W2023954727 cites W2076669652 @default.
• W2023954727 cites W2077400503 @default.
• W2023954727 cites W2094927492 @default.
• W2023954727 cites W2099616208 @default.
• W2023954727 cites W2107450430 @default.
• W2023954727 cites W2130647505 @default.
• W2023954727 cites W2147108123 @default.
• W2023954727 cites W2161077668 @default.
• W2023954727 cites W2183266848 @default.
• W2023954727 cites W2320389941 @default.
• W2023954727 doi "https://doi.org/10.1263/jbb.102.102" @default.
• W2023954727 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17027871" @default.
• W2023954727 hasPublicationYear "2006" @default.
• W2023954727 type Work @default.
• W2023954727 sameAs 2023954727 @default.
• W2023954727 citedByCount "14" @default.
• W2023954727 countsByYear W20239547272012 @default.
• W2023954727 countsByYear W20239547272013 @default.
• W2023954727 countsByYear W20239547272014 @default.
• W2023954727 countsByYear W20239547272015 @default.
• W2023954727 countsByYear W20239547272018 @default.
• W2023954727 countsByYear W20239547272021 @default.
• W2023954727 crossrefType "journal-article" @default.
• W2023954727 hasAuthorship W2023954727A5002406091 @default.
• W2023954727 hasAuthorship W2023954727A5023419492 @default.
• W2023954727 hasAuthorship W2023954727A5025070827 @default.
• W2023954727 hasAuthorship W2023954727A5034075544 @default.
• W2023954727 hasAuthorship W2023954727A5079582179 @default.
• W2023954727 hasAuthorship W2023954727A5090298115 @default.
• W2023954727 hasConcept C104317684 @default.
• W2023954727 hasConcept C10858879 @default.
• W2023954727 hasConcept C119062480 @default.
• W2023954727 hasConcept C153911025 @default.
• W2023954727 hasConcept C156399914 @default.
• W2023954727 hasConcept C158617107 @default.
• W2023954727 hasConcept C162008176 @default.
• W2023954727 hasConcept C181199279 @default.
• W2023954727 hasConcept C185592680 @default.
• W2023954727 hasConcept C2777676563 @default.
• W2023954727 hasConcept C2781205006 @default.
• W2023954727 hasConcept C40767141 @default.
• W2023954727 hasConcept C49039625 @default.
• W2023954727 hasConcept C55493867 @default.
• W2023954727 hasConcept C6492254 @default.
• W2023954727 hasConcept C7860815 @default.
• W2023954727 hasConcept C86803240 @default.
• W2023954727 hasConceptScore W2023954727C104317684 @default.
• W2023954727 hasConceptScore W2023954727C10858879 @default.
• W2023954727 hasConceptScore W2023954727C119062480 @default.
• W2023954727 hasConceptScore W2023954727C153911025 @default.
• W2023954727 hasConceptScore W2023954727C156399914 @default.
• W2023954727 hasConceptScore W2023954727C158617107 @default.
• W2023954727 hasConceptScore W2023954727C162008176 @default.
• W2023954727 hasConceptScore W2023954727C181199279 @default.
• W2023954727 hasConceptScore W2023954727C185592680 @default.
• W2023954727 hasConceptScore W2023954727C2777676563 @default.
• W2023954727 hasConceptScore W2023954727C2781205006 @default.
• W2023954727 hasConceptScore W2023954727C40767141 @default.
• W2023954727 hasConceptScore W2023954727C49039625 @default.
• W2023954727 hasConceptScore W2023954727C55493867 @default.
• W2023954727 hasConceptScore W2023954727C6492254 @default.
• W2023954727 hasConceptScore W2023954727C7860815 @default.
• W2023954727 hasConceptScore W2023954727C86803240 @default.

• next