FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023963622> ?p ?o ?g. }

• W2023963622 endingPage "2559" @default.
• W2023963622 startingPage "2554" @default.
• W2023963622 abstract "Conventional DNA sequencing is based on gel electrophoretic separation of the sequencing products. Gel casting and electrophoresis are the time limiting steps, and the gel separation is occasionally imperfect due to aberrant mobility of certain fragments, leading to erroneous sequence determination. Furthermore, illegitimately terminated products frequently cannot be distinguished from correctly terminated ones, a phenomenon that also obscures data interpretation. In the present work the use of MALDI mass spectrometry for sequencing of DNA amplified from clinical samples is implemented. The unambiguous and fast identification of deletions and substitutions in DNA amplified from heterozygous carriers realistically suggest MALDI mass spectrometry as a future alternative to conventional sequencing procedures for high throughput screening for mutations. Unique features of the method are demonstrated by sequencing a DNA fragment that could not be sequenced conventionally because of gel electrophoretic band compression and the presence of multiple non-specific termination products. Taking advantage of the accurate mass information provided by MALDI mass spectrometry, the sequence was deduced, and the nature of the non-specific termination could be determined. The method described here increases the fidelity in DNA sequencing, is fast, compatible with standard DNA sequencing procedures, and amenable to automation." @default.
• W2023963622 created "2016-06-24" @default.
• W2023963622 creator A5009458005 @default.
• W2023963622 creator A5032485134 @default.
• W2023963622 creator A5049027923 @default.
• W2023963622 creator A5051563723 @default.
• W2023963622 creator A5072803823 @default.
• W2023963622 creator A5091865923 @default.
• W2023963622 date "1998-06-01" @default.
• W2023963622 modified "2023-10-10" @default.
• W2023963622 title "DNA sequence analysis by MALDI mass spectrometry" @default.
• W2023963622 cites W1806652799 @default.
• W2023963622 cites W1965229422 @default.
• W2023963622 cites W1973034108 @default.
• W2023963622 cites W1973529204 @default.
• W2023963622 cites W1974830441 @default.
• W2023963622 cites W1978349009 @default.
• W2023963622 cites W1989138168 @default.
• W2023963622 cites W2004416970 @default.
• W2023963622 cites W2014323182 @default.
• W2023963622 cites W2021852038 @default.
• W2023963622 cites W2025735321 @default.
• W2023963622 cites W2040033652 @default.
• W2023963622 cites W2047898183 @default.
• W2023963622 cites W2053090704 @default.
• W2023963622 cites W2063936868 @default.
• W2023963622 cites W2145839720 @default.
• W2023963622 cites W2148023601 @default.
• W2023963622 cites W2154296702 @default.
• W2023963622 cites W2004436917 @default.
• W2023963622 doi "https://doi.org/10.1093/nar/26.11.2554" @default.
• W2023963622 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/147593" @default.
• W2023963622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9592136" @default.
• W2023963622 hasPublicationYear "1998" @default.
• W2023963622 type Work @default.
• W2023963622 sameAs 2023963622 @default.
• W2023963622 citedByCount "109" @default.
• W2023963622 countsByYear W20239636222012 @default.
• W2023963622 countsByYear W20239636222013 @default.
• W2023963622 countsByYear W20239636222014 @default.
• W2023963622 countsByYear W20239636222015 @default.
• W2023963622 countsByYear W20239636222016 @default.
• W2023963622 countsByYear W20239636222017 @default.
• W2023963622 countsByYear W20239636222018 @default.
• W2023963622 countsByYear W20239636222019 @default.
• W2023963622 countsByYear W20239636222020 @default.
• W2023963622 countsByYear W20239636222021 @default.
• W2023963622 countsByYear W20239636222022 @default.
• W2023963622 crossrefType "journal-article" @default.
• W2023963622 hasAuthorship W2023963622A5009458005 @default.
• W2023963622 hasAuthorship W2023963622A5032485134 @default.
• W2023963622 hasAuthorship W2023963622A5049027923 @default.
• W2023963622 hasAuthorship W2023963622A5051563723 @default.
• W2023963622 hasAuthorship W2023963622A5072803823 @default.
• W2023963622 hasAuthorship W2023963622A5091865923 @default.
• W2023963622 hasBestOaLocation W20239636221 @default.
• W2023963622 hasConcept C116084860 @default.
• W2023963622 hasConcept C129312508 @default.
• W2023963622 hasConcept C13575015 @default.
• W2023963622 hasConcept C162356407 @default.
• W2023963622 hasConcept C185592680 @default.
• W2023963622 hasConcept C3017666073 @default.
• W2023963622 hasConcept C43617362 @default.
• W2023963622 hasConcept C51679486 @default.
• W2023963622 hasConcept C54355233 @default.
• W2023963622 hasConcept C54887055 @default.
• W2023963622 hasConcept C552990157 @default.
• W2023963622 hasConcept C70721500 @default.
• W2023963622 hasConcept C86803240 @default.
• W2023963622 hasConceptScore W2023963622C116084860 @default.
• W2023963622 hasConceptScore W2023963622C129312508 @default.
• W2023963622 hasConceptScore W2023963622C13575015 @default.
• W2023963622 hasConceptScore W2023963622C162356407 @default.
• W2023963622 hasConceptScore W2023963622C185592680 @default.
• W2023963622 hasConceptScore W2023963622C3017666073 @default.
• W2023963622 hasConceptScore W2023963622C43617362 @default.
• W2023963622 hasConceptScore W2023963622C51679486 @default.
• W2023963622 hasConceptScore W2023963622C54355233 @default.
• W2023963622 hasConceptScore W2023963622C54887055 @default.
• W2023963622 hasConceptScore W2023963622C552990157 @default.
• W2023963622 hasConceptScore W2023963622C70721500 @default.
• W2023963622 hasConceptScore W2023963622C86803240 @default.
• W2023963622 hasIssue "11" @default.
• W2023963622 hasLocation W20239636221 @default.
• W2023963622 hasLocation W20239636222 @default.
• W2023963622 hasLocation W20239636223 @default.
• W2023963622 hasLocation W20239636224 @default.
• W2023963622 hasOpenAccess W2023963622 @default.
• W2023963622 hasPrimaryLocation W20239636221 @default.
• W2023963622 hasRelatedWork W1593660693 @default.
• W2023963622 hasRelatedWork W2029446026 @default.
• W2023963622 hasRelatedWork W2059362154 @default.
• W2023963622 hasRelatedWork W2070479548 @default.
• W2023963622 hasRelatedWork W2134447998 @default.
• W2023963622 hasRelatedWork W2395675319 @default.
• W2023963622 hasRelatedWork W2418987332 @default.
• W2023963622 hasRelatedWork W2782499911 @default.
• W2023963622 hasRelatedWork W4248897355 @default.

• next