FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023971680> ?p ?o ?g. }

• W2023971680 endingPage "422" @default.
• W2023971680 startingPage "415" @default.
• W2023971680 abstract "The present study reports a successful culture system of rat bone cells obtained from the bare long bone fragments of neonatal Wistar rats. The morphology, histochemical and immunocytochemical properties of the cultured cells were characterized by phase contrast microscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), histochemical staining with alkaline phosphatase (ALP), and immunocytochemical staining with the anti-osteocalcin antibodies. Under phase contrast microscopy, the cultured rat bone cells changed their morphology from being slender with relatively-short cytoplasmic processes to becoming polygonal with longer processes, and subsequently flattened. These cultured bone cells formed bone nodules after 3 weeks. Under SEM, the cultured bone cell appeared polygonal with some microvilli. The TEM showed that their cytoplasm contained abundant endoplasmic reticulum and well-developed Golgi apparatus. Positive histochemical staining of ALP was also detected as a blue coloration and granular appearance in the cultured bone cells. Immunocytochemistry with the polyclonal and monoclonal anti-osteocalcin antibodies showed the localization of osteocalcin in both the cytoplasm and nucleus. All these data lead us to consider that the cultured bone cells in our system were probably osteoblasts from growing bone tissue in vitro. This provides a convenient test system for further studies on the regulation of the growth and metabolism of rat bone cells." @default.
• W2023971680 created "2016-06-24" @default.
• W2023971680 creator A5009738321 @default.
• W2023971680 creator A5032949945 @default.
• W2023971680 creator A5041417481 @default.
• W2023971680 creator A5054073903 @default.
• W2023971680 creator A5086908850 @default.
• W2023971680 date "1992-01-01" @default.
• W2023971680 modified "2023-09-26" @default.
• W2023971680 title "Morphological and Immunocytochemical Characterization of Osteoblast Cultures from Long Bones of Neonatal Rats." @default.
• W2023971680 cites W1507107364 @default.
• W2023971680 cites W1508172213 @default.
• W2023971680 cites W1574632723 @default.
• W2023971680 cites W1967882105 @default.
• W2023971680 cites W1976030527 @default.
• W2023971680 cites W1987495895 @default.
• W2023971680 cites W1990572325 @default.
• W2023971680 cites W1991432123 @default.
• W2023971680 cites W2003022060 @default.
• W2023971680 cites W2003523753 @default.
• W2023971680 cites W2004852171 @default.
• W2023971680 cites W2021563374 @default.
• W2023971680 cites W2041507165 @default.
• W2023971680 cites W2049939093 @default.
• W2023971680 cites W2068596665 @default.
• W2023971680 cites W2070194131 @default.
• W2023971680 cites W2086239373 @default.
• W2023971680 cites W2088803535 @default.
• W2023971680 cites W2090181441 @default.
• W2023971680 cites W2157728558 @default.
• W2023971680 cites W2407455852 @default.
• W2023971680 cites W2461263194 @default.
• W2023971680 cites W340927772 @default.
• W2023971680 doi "https://doi.org/10.1679/aohc.55.415" @default.
• W2023971680 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1482606" @default.
• W2023971680 hasPublicationYear "1992" @default.
• W2023971680 type Work @default.
• W2023971680 sameAs 2023971680 @default.
• W2023971680 citedByCount "5" @default.
• W2023971680 crossrefType "journal-article" @default.
• W2023971680 hasAuthorship W2023971680A5009738321 @default.
• W2023971680 hasAuthorship W2023971680A5032949945 @default.
• W2023971680 hasAuthorship W2023971680A5041417481 @default.
• W2023971680 hasAuthorship W2023971680A5054073903 @default.
• W2023971680 hasAuthorship W2023971680A5086908850 @default.
• W2023971680 hasBestOaLocation W20239716801 @default.
• W2023971680 hasConcept C105702510 @default.
• W2023971680 hasConcept C119062480 @default.
• W2023971680 hasConcept C131075544 @default.
• W2023971680 hasConcept C133605539 @default.
• W2023971680 hasConcept C134018914 @default.
• W2023971680 hasConcept C142724271 @default.
• W2023971680 hasConcept C158617107 @default.
• W2023971680 hasConcept C160160445 @default.
• W2023971680 hasConcept C181199279 @default.
• W2023971680 hasConcept C185592680 @default.
• W2023971680 hasConcept C190062978 @default.
• W2023971680 hasConcept C202751555 @default.
• W2023971680 hasConcept C2778260815 @default.
• W2023971680 hasConcept C35866371 @default.
• W2023971680 hasConcept C54355233 @default.
• W2023971680 hasConcept C55493867 @default.
• W2023971680 hasConcept C71924100 @default.
• W2023971680 hasConcept C74864618 @default.
• W2023971680 hasConcept C81880429 @default.
• W2023971680 hasConcept C86803240 @default.
• W2023971680 hasConcept C95444343 @default.
• W2023971680 hasConceptScore W2023971680C105702510 @default.
• W2023971680 hasConceptScore W2023971680C119062480 @default.
• W2023971680 hasConceptScore W2023971680C131075544 @default.
• W2023971680 hasConceptScore W2023971680C133605539 @default.
• W2023971680 hasConceptScore W2023971680C134018914 @default.
• W2023971680 hasConceptScore W2023971680C142724271 @default.
• W2023971680 hasConceptScore W2023971680C158617107 @default.
• W2023971680 hasConceptScore W2023971680C160160445 @default.
• W2023971680 hasConceptScore W2023971680C181199279 @default.
• W2023971680 hasConceptScore W2023971680C185592680 @default.
• W2023971680 hasConceptScore W2023971680C190062978 @default.
• W2023971680 hasConceptScore W2023971680C202751555 @default.
• W2023971680 hasConceptScore W2023971680C2778260815 @default.
• W2023971680 hasConceptScore W2023971680C35866371 @default.
• W2023971680 hasConceptScore W2023971680C54355233 @default.
• W2023971680 hasConceptScore W2023971680C55493867 @default.
• W2023971680 hasConceptScore W2023971680C71924100 @default.
• W2023971680 hasConceptScore W2023971680C74864618 @default.
• W2023971680 hasConceptScore W2023971680C81880429 @default.
• W2023971680 hasConceptScore W2023971680C86803240 @default.
• W2023971680 hasConceptScore W2023971680C95444343 @default.
• W2023971680 hasIssue "4" @default.
• W2023971680 hasLocation W20239716801 @default.
• W2023971680 hasLocation W20239716802 @default.
• W2023971680 hasOpenAccess W2023971680 @default.
• W2023971680 hasPrimaryLocation W20239716801 @default.
• W2023971680 hasRelatedWork W1986368683 @default.
• W2023971680 hasRelatedWork W1988750237 @default.
• W2023971680 hasRelatedWork W2023545448 @default.
• W2023971680 hasRelatedWork W2044971600 @default.
• W2023971680 hasRelatedWork W2055634467 @default.

• next