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• W2023981782 endingPage "1975" @default.
• W2023981782 startingPage "1971" @default.
• W2023981782 abstract "Both polarities of the satellite RNA of tobacco ringspot virus occur in infected cells in multimeric forms which are capable of autolytic processing, using different sequences and structures [Feldstein, P.A., et al., Proc. Nat. Acad. Sci. USA (1990) 87 (in press)]. These transesterification reactions generate a 2',3'-cyclophosphate and a 5'-hydroxyl as the two new end groups. Cleavage is at a CpA for the (+) polarity RNA and at an ApG for the (-) polarity RNA. We enzymically synthesized oligoribonucleotides with processing capability and with specific 35S-labeled phosphorothioate diesters in the Rp configuration. After processing had occurred, the terminal nucleoside-2',3'-cyclophosphorothioate diester residues were recovered from the appropriate product by digestion with nuclease and phosphatase. Comparisons with specially prepared endo- and exoisomer reference compounds by thin layer chromatography and autoradiography revealed that the [35S]cytidine- and [35S]adenosine-2',3'-cyclophosphorothioate both were endo-isomers. The results are consistent with transesterification occurring by an inline SN2(P) attack of the 2'-hydroxyl group in the autolytic processing reactions of both polarities of the satellite RNA." @default.
• W2023981782 created "2016-06-24" @default.
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• W2023981782 date "1990-01-01" @default.
• W2023981782 modified "2023-09-26" @default.
• W2023981782 title "Two autolytic processing reactions of a satellite RNA proceed with inversion of configuration" @default.
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• W2023981782 doi "https://doi.org/10.1093/nar/18.8.1971" @default.
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