Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023986557> ?p ?o ?g. }
- W2023986557 endingPage "10677" @default.
- W2023986557 startingPage "10666" @default.
- W2023986557 abstract "SlyD is a Ni(II)-binding protein that contributes to nickel homeostasis in Escherichia coli. The C-terminal domain of SlyD contains a rich variety of metal-binding amino acids, suggesting broader metal binding capabilities, and previous work demonstrated that the protein can coordinate several types of first-row transition metals. However, the binding of SlyD to metals other than Ni(II) has not been previously characterized. To improve our understanding of the in vitro metal-binding activity of SlyD and how it correlates with the in vivo function of this protein, the interactions between SlyD and the series of biologically relevant transition metals [Mn(II), Fe(II), Co(II), Cu(I), and Zn(II)] were examined by using a combination of optical spectroscopy and mass spectrometry. Binding of SlyD to Mn(II) or Fe(II) ions was not detected, but the protein coordinates multiple ions of Co(II), Zn(II), and Cu(I) with appreciable affinity (KD values in or below the nanomolar range), highlighting the promiscuous nature of this protein. The order of affinities of SlyD for the metals examined is as follows: Mn(II) and Fe(II) < Co(II) < Ni(II) ∼ Zn(II) ≪ Cu(I). Although the purified protein is unable to overcome the large thermodynamic preference for Cu(I) and exclude Zn(II) chelation in the presence of Ni(II), in vivo studies reveal a Ni(II)-specific function for the protein. Furthermore, these latter experiments support a specific role for SlyD as a [NiFe]-hydrogenase enzyme maturation factor. The implications of the divergence between the metal selectivity of SlyD in vitro and the specific activity in vivo are discussed." @default.
- W2023986557 created "2016-06-24" @default.
- W2023986557 creator A5009769045 @default.
- W2023986557 creator A5013481163 @default.
- W2023986557 creator A5013986691 @default.
- W2023986557 creator A5015829651 @default.
- W2023986557 creator A5034517384 @default.
- W2023986557 creator A5049226429 @default.
- W2023986557 creator A5083714655 @default.
- W2023986557 date "2011-11-14" @default.
- W2023986557 modified "2023-09-30" @default.
- W2023986557 title "Metal Selectivity of the <i>Escherichia coli</i> Nickel Metallochaperone, SlyD" @default.
- W2023986557 cites W1490783082 @default.
- W2023986557 cites W1550809462 @default.
- W2023986557 cites W1563527550 @default.
- W2023986557 cites W1977536637 @default.
- W2023986557 cites W1977557707 @default.
- W2023986557 cites W1979289669 @default.
- W2023986557 cites W1979488926 @default.
- W2023986557 cites W1980099457 @default.
- W2023986557 cites W1986080062 @default.
- W2023986557 cites W1992365072 @default.
- W2023986557 cites W1995036572 @default.
- W2023986557 cites W1995732138 @default.
- W2023986557 cites W2004453869 @default.
- W2023986557 cites W2005961039 @default.
- W2023986557 cites W2011648574 @default.
- W2023986557 cites W2015806444 @default.
- W2023986557 cites W2017980572 @default.
- W2023986557 cites W2023026110 @default.
- W2023986557 cites W2023089183 @default.
- W2023986557 cites W2029240756 @default.
- W2023986557 cites W2031294010 @default.
- W2023986557 cites W2034756580 @default.
- W2023986557 cites W2034847587 @default.
- W2023986557 cites W2035594105 @default.
- W2023986557 cites W2038859671 @default.
- W2023986557 cites W2041951808 @default.
- W2023986557 cites W2044984846 @default.
- W2023986557 cites W2048985128 @default.
- W2023986557 cites W2058170418 @default.
- W2023986557 cites W2064212116 @default.
- W2023986557 cites W2067578088 @default.
- W2023986557 cites W2082908227 @default.
- W2023986557 cites W2084168858 @default.
- W2023986557 cites W2089495822 @default.
- W2023986557 cites W2090196510 @default.
- W2023986557 cites W2092350661 @default.
- W2023986557 cites W2094256135 @default.
- W2023986557 cites W2096976702 @default.
- W2023986557 cites W2105786325 @default.
- W2023986557 cites W2111967267 @default.
- W2023986557 cites W2113464808 @default.
- W2023986557 cites W2121417699 @default.
- W2023986557 cites W2134640814 @default.
- W2023986557 cites W2141260882 @default.
- W2023986557 cites W2144070276 @default.
- W2023986557 cites W2153001370 @default.
- W2023986557 cites W2154159254 @default.
- W2023986557 cites W2155100129 @default.
- W2023986557 cites W2161407287 @default.
- W2023986557 cites W2165658854 @default.
- W2023986557 cites W2166896483 @default.
- W2023986557 cites W2952643498 @default.
- W2023986557 doi "https://doi.org/10.1021/bi2014882" @default.
- W2023986557 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3253375" @default.
- W2023986557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22047179" @default.
- W2023986557 hasPublicationYear "2011" @default.
- W2023986557 type Work @default.
- W2023986557 sameAs 2023986557 @default.
- W2023986557 citedByCount "18" @default.
- W2023986557 countsByYear W20239865572012 @default.
- W2023986557 countsByYear W20239865572013 @default.
- W2023986557 countsByYear W20239865572014 @default.
- W2023986557 countsByYear W20239865572015 @default.
- W2023986557 countsByYear W20239865572016 @default.
- W2023986557 countsByYear W20239865572017 @default.
- W2023986557 countsByYear W20239865572020 @default.
- W2023986557 countsByYear W20239865572021 @default.
- W2023986557 crossrefType "journal-article" @default.
- W2023986557 hasAuthorship W2023986557A5009769045 @default.
- W2023986557 hasAuthorship W2023986557A5013481163 @default.
- W2023986557 hasAuthorship W2023986557A5013986691 @default.
- W2023986557 hasAuthorship W2023986557A5015829651 @default.
- W2023986557 hasAuthorship W2023986557A5034517384 @default.
- W2023986557 hasAuthorship W2023986557A5049226429 @default.
- W2023986557 hasAuthorship W2023986557A5083714655 @default.
- W2023986557 hasBestOaLocation W20239865572 @default.
- W2023986557 hasConcept C106773901 @default.
- W2023986557 hasConcept C161790260 @default.
- W2023986557 hasConcept C17165728 @default.
- W2023986557 hasConcept C178790620 @default.
- W2023986557 hasConcept C185592680 @default.
- W2023986557 hasConcept C2780283098 @default.
- W2023986557 hasConcept C504270822 @default.
- W2023986557 hasConcept C544153396 @default.
- W2023986557 hasConcept C55493867 @default.
- W2023986557 hasConcept C71240020 @default.