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• W2023994677 abstract "Two sequence-verified, clonal, publicly available collections of human open reading frames are reported. One collection is in a lentiviral vector for expression in mammalian cells; the other is in the Gateway vector system. Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types." @default.
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• W2023994677 date "2011-06-26" @default.
• W2023994677 modified "2023-10-01" @default.
• W2023994677 title "A public genome-scale lentiviral expression library of human ORFs" @default.
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• W2023994677 doi "https://doi.org/10.1038/nmeth.1638" @default.
• W2023994677 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3234135" @default.
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