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• W2023996342 abstract "PEEK-WC-PU membranes were modified with an NH(3) glow discharge process to graft N-containing functional groups at their surface in order to improve the maintenance of human hepatocytes. Native and modified membrane surfaces were characterized with XPS, ToF-SIMS and WCA measurements. We have investigated morphological behaviour and specific functions of primary human hepatocytes on native and modified PEEK-WC-PU membranes in a small-scale gas-permeable bioreactor. N-containing groups grafted at the surface of the membranes improved the initial steps of adhesion and the maintenance of phenotype and differentiated functions of cells. Confocal microscopy of cell morphology evidenced human hepatocytes exhibiting a polygonal shape and organizing a 3D structure. The presence of CK19 positive cells, a marker of biliary duct epithelium, was also found on native and modified membranes. Liver specific functions, investigated in terms of urea production, albumin synthesis and diazepam biotransformation, were maintained at high levels up to 19 days, particularly on surface-modified membranes." @default.
• W2023996342 created "2016-06-24" @default.
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• W2023996342 date "2009-09-01" @default.
• W2023996342 modified "2023-09-23" @default.
• W2023996342 title "Improved functions of human hepatocytes on NH3 plasma-grafted PEEK-WC–PU membranes" @default.
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• W2023996342 doi "https://doi.org/10.1016/j.biomaterials.2009.04.052" @default.
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• W2023996342 hasPublicationYear "2009" @default.
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