FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023996602> ?p ?o ?g. }

• W2023996602 abstract "MicroRNAs regulate gene expression at the post-transcriptional level. Differential expression of miRNAs can potentially be used as biomarkers for early diagnosis and prediction for outcomes. Failure in validation of miRNA profiles is often caused by variations in experimental parameters. In this study, the performance of five extraction kits and three RT-qPCR systems were evaluated using BioMark high-throughput platform and the effects of different experimental parameters on circulating miRNA levels were determined. Differences in the performance of extraction kits as well as varying accuracy, sensitivity and reproducibility in qPCR systems were observed. Normalisation of RT-qPCR data to spike-in controls can reduce extraction bias. However, the extent of correlation for different qPCR systems varies in different assays. At different time points, there was no significant fold change in eight of the plasma miRNAs that we evaluated. Higher level of miRNAs was detected in plasma as compared to serum of the same cohort. In summary, we demonstrated that high-throughput RT-qPCR with pre-amplification step had increased sensitivity and can be achieved with accuracy and high reproducibility through stringent experimental controls. The information provided here is useful for planning biomarker validation studies involving circulating miRNAs." @default.
• W2023996602 created "2016-06-24" @default.
• W2023996602 creator A5007639658 @default.
• W2023996602 creator A5018548511 @default.
• W2023996602 creator A5064396695 @default.
• W2023996602 date "2015-03-24" @default.
• W2023996602 modified "2023-10-13" @default.
• W2023996602 title "Evaluation of extraction kits and RT-qPCR systems adapted to high-throughput platform for circulating miRNAs" @default.
• W2023996602 cites W144423133 @default.
• W2023996602 cites W1584433563 @default.
• W2023996602 cites W1968631483 @default.
• W2023996602 cites W1971726501 @default.
• W2023996602 cites W1982810690 @default.
• W2023996602 cites W1983932066 @default.
• W2023996602 cites W1992967330 @default.
• W2023996602 cites W2001358823 @default.
• W2023996602 cites W2007800006 @default.
• W2023996602 cites W2008594900 @default.
• W2023996602 cites W2012536934 @default.
• W2023996602 cites W2021560851 @default.
• W2023996602 cites W2032696674 @default.
• W2023996602 cites W2040404560 @default.
• W2023996602 cites W2054703855 @default.
• W2023996602 cites W2074689365 @default.
• W2023996602 cites W2092913756 @default.
• W2023996602 cites W2094421850 @default.
• W2023996602 cites W2104607843 @default.
• W2023996602 cites W2115093090 @default.
• W2023996602 cites W2119604948 @default.
• W2023996602 cites W2132688909 @default.
• W2023996602 cites W2133365560 @default.
• W2023996602 cites W2135617615 @default.
• W2023996602 cites W2135977037 @default.
• W2023996602 cites W2140775205 @default.
• W2023996602 cites W2149966907 @default.
• W2023996602 cites W2152891840 @default.
• W2023996602 cites W2159627472 @default.
• W2023996602 cites W2163482204 @default.
• W2023996602 cites W2166273484 @default.
• W2023996602 doi "https://doi.org/10.1038/srep09430" @default.
• W2023996602 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4371102" @default.
• W2023996602 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25800946" @default.
• W2023996602 hasPublicationYear "2015" @default.
• W2023996602 type Work @default.
• W2023996602 sameAs 2023996602 @default.
• W2023996602 citedByCount "49" @default.
• W2023996602 countsByYear W20239966022015 @default.
• W2023996602 countsByYear W20239966022016 @default.
• W2023996602 countsByYear W20239966022017 @default.
• W2023996602 countsByYear W20239966022018 @default.
• W2023996602 countsByYear W20239966022019 @default.
• W2023996602 countsByYear W20239966022020 @default.
• W2023996602 countsByYear W20239966022021 @default.
• W2023996602 countsByYear W20239966022022 @default.
• W2023996602 countsByYear W20239966022023 @default.
• W2023996602 crossrefType "journal-article" @default.
• W2023996602 hasAuthorship W2023996602A5007639658 @default.
• W2023996602 hasAuthorship W2023996602A5018548511 @default.
• W2023996602 hasAuthorship W2023996602A5064396695 @default.
• W2023996602 hasBestOaLocation W20239966021 @default.
• W2023996602 hasConcept C104317684 @default.
• W2023996602 hasConcept C112190416 @default.
• W2023996602 hasConcept C145059251 @default.
• W2023996602 hasConcept C150194340 @default.
• W2023996602 hasConcept C157764524 @default.
• W2023996602 hasConcept C185592680 @default.
• W2023996602 hasConcept C2781197716 @default.
• W2023996602 hasConcept C41008148 @default.
• W2023996602 hasConcept C43617362 @default.
• W2023996602 hasConcept C4725764 @default.
• W2023996602 hasConcept C48023723 @default.
• W2023996602 hasConcept C54355233 @default.
• W2023996602 hasConcept C555944384 @default.
• W2023996602 hasConcept C60644358 @default.
• W2023996602 hasConcept C70721500 @default.
• W2023996602 hasConcept C76155785 @default.
• W2023996602 hasConcept C86803240 @default.
• W2023996602 hasConcept C9893847 @default.
• W2023996602 hasConceptScore W2023996602C104317684 @default.
• W2023996602 hasConceptScore W2023996602C112190416 @default.
• W2023996602 hasConceptScore W2023996602C145059251 @default.
• W2023996602 hasConceptScore W2023996602C150194340 @default.
• W2023996602 hasConceptScore W2023996602C157764524 @default.
• W2023996602 hasConceptScore W2023996602C185592680 @default.
• W2023996602 hasConceptScore W2023996602C2781197716 @default.
• W2023996602 hasConceptScore W2023996602C41008148 @default.
• W2023996602 hasConceptScore W2023996602C43617362 @default.
• W2023996602 hasConceptScore W2023996602C4725764 @default.
• W2023996602 hasConceptScore W2023996602C48023723 @default.
• W2023996602 hasConceptScore W2023996602C54355233 @default.
• W2023996602 hasConceptScore W2023996602C555944384 @default.
• W2023996602 hasConceptScore W2023996602C60644358 @default.
• W2023996602 hasConceptScore W2023996602C70721500 @default.
• W2023996602 hasConceptScore W2023996602C76155785 @default.
• W2023996602 hasConceptScore W2023996602C86803240 @default.
• W2023996602 hasConceptScore W2023996602C9893847 @default.
• W2023996602 hasIssue "1" @default.
• W2023996602 hasLocation W20239966021 @default.
• W2023996602 hasLocation W20239966022 @default.
• W2023996602 hasLocation W20239966023 @default.

• next