Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023997802> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2023997802 endingPage "549" @default.
- W2023997802 startingPage "543" @default.
- W2023997802 abstract "The neurotoxic activity of ammodytoxin A (AtxA), a phospholipase A(2) from Vipera ammodytes ammodytes venom, has been investigated by protein engineering. With the aim of obtaining AtxA as a non-fused protein in the bacterial cytoplasm and avoiding problems with incomplete cleavage in vivo of the initial Met preceding the first residue (Ser1), a double mutant (S1A/E4Q) was prepared and expressed in Escherichia coli. Immunoblotting of the bacterial lysate showed that the mutant was synthesized at a low level not exceeding 0.5% of total cell protein. Analysis of the potential secondary structure of the mutant mRNA in the translation initiation region suggested that the Ala1 (GCC) and Leu2 (CUG) codons used are likely to be involved in a hairpin structure with the Thr13 (ACG) and Gly14 (GGG) codons, hindering effective translation at the ribosome. To weaken this structure (by DeltaG of about 20 kJ/mol) the same double mutant was prepared using another mutagenic oligonucleotide with silent mutations in the Ala1 (GCU) and Leu2 (UUG) codons. The mutant was successfully produced at a level of approximately 15% of total protein, with the initial Met completely removed in the bacterial cell. Such an approach could be important in solving similar problems in bacterial production of other toxic proteins." @default.
- W2023997802 created "2016-06-24" @default.
- W2023997802 creator A5004552048 @default.
- W2023997802 creator A5010893774 @default.
- W2023997802 creator A5034928625 @default.
- W2023997802 date "2002-05-01" @default.
- W2023997802 modified "2023-10-18" @default.
- W2023997802 title "mRNA secondary structure can greatly affect production of recombinant phospholipase A2 toxins in bacteria" @default.
- W2023997802 cites W141039805 @default.
- W2023997802 cites W1711078594 @default.
- W2023997802 cites W1873199501 @default.
- W2023997802 cites W1968985054 @default.
- W2023997802 cites W1993523153 @default.
- W2023997802 cites W2009818318 @default.
- W2023997802 cites W2016780222 @default.
- W2023997802 cites W2037909798 @default.
- W2023997802 cites W2038503882 @default.
- W2023997802 cites W2062837040 @default.
- W2023997802 cites W2073354904 @default.
- W2023997802 cites W2080388832 @default.
- W2023997802 cites W2083842949 @default.
- W2023997802 cites W2090196514 @default.
- W2023997802 cites W2093396859 @default.
- W2023997802 cites W2138270253 @default.
- W2023997802 cites W2139217585 @default.
- W2023997802 cites W2170602689 @default.
- W2023997802 cites W4317926880 @default.
- W2023997802 doi "https://doi.org/10.1016/s0041-0101(01)00250-1" @default.
- W2023997802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11821126" @default.
- W2023997802 hasPublicationYear "2002" @default.
- W2023997802 type Work @default.
- W2023997802 sameAs 2023997802 @default.
- W2023997802 citedByCount "11" @default.
- W2023997802 countsByYear W20239978022018 @default.
- W2023997802 crossrefType "journal-article" @default.
- W2023997802 hasAuthorship W2023997802A5004552048 @default.
- W2023997802 hasAuthorship W2023997802A5010893774 @default.
- W2023997802 hasAuthorship W2023997802A5034928625 @default.
- W2023997802 hasConcept C104317684 @default.
- W2023997802 hasConcept C105580179 @default.
- W2023997802 hasConcept C114847915 @default.
- W2023997802 hasConcept C143065580 @default.
- W2023997802 hasConcept C149364088 @default.
- W2023997802 hasConcept C153911025 @default.
- W2023997802 hasConcept C176990463 @default.
- W2023997802 hasConcept C185592680 @default.
- W2023997802 hasConcept C2779926675 @default.
- W2023997802 hasConcept C3675279 @default.
- W2023997802 hasConcept C4718897 @default.
- W2023997802 hasConcept C547475151 @default.
- W2023997802 hasConcept C55493867 @default.
- W2023997802 hasConcept C57409179 @default.
- W2023997802 hasConcept C67705224 @default.
- W2023997802 hasConcept C86803240 @default.
- W2023997802 hasConcept C88478588 @default.
- W2023997802 hasConceptScore W2023997802C104317684 @default.
- W2023997802 hasConceptScore W2023997802C105580179 @default.
- W2023997802 hasConceptScore W2023997802C114847915 @default.
- W2023997802 hasConceptScore W2023997802C143065580 @default.
- W2023997802 hasConceptScore W2023997802C149364088 @default.
- W2023997802 hasConceptScore W2023997802C153911025 @default.
- W2023997802 hasConceptScore W2023997802C176990463 @default.
- W2023997802 hasConceptScore W2023997802C185592680 @default.
- W2023997802 hasConceptScore W2023997802C2779926675 @default.
- W2023997802 hasConceptScore W2023997802C3675279 @default.
- W2023997802 hasConceptScore W2023997802C4718897 @default.
- W2023997802 hasConceptScore W2023997802C547475151 @default.
- W2023997802 hasConceptScore W2023997802C55493867 @default.
- W2023997802 hasConceptScore W2023997802C57409179 @default.
- W2023997802 hasConceptScore W2023997802C67705224 @default.
- W2023997802 hasConceptScore W2023997802C86803240 @default.
- W2023997802 hasConceptScore W2023997802C88478588 @default.
- W2023997802 hasFunder F4320323569 @default.
- W2023997802 hasIssue "5" @default.
- W2023997802 hasLocation W20239978021 @default.
- W2023997802 hasLocation W20239978022 @default.
- W2023997802 hasOpenAccess W2023997802 @default.
- W2023997802 hasPrimaryLocation W20239978021 @default.
- W2023997802 hasRelatedWork W1973198873 @default.
- W2023997802 hasRelatedWork W1997085343 @default.
- W2023997802 hasRelatedWork W2041030563 @default.
- W2023997802 hasRelatedWork W2123299226 @default.
- W2023997802 hasRelatedWork W2419044071 @default.
- W2023997802 hasRelatedWork W2589945527 @default.
- W2023997802 hasRelatedWork W2949261036 @default.
- W2023997802 hasRelatedWork W3042772396 @default.
- W2023997802 hasRelatedWork W3172142859 @default.
- W2023997802 hasRelatedWork W4288858747 @default.
- W2023997802 hasVolume "40" @default.
- W2023997802 isParatext "false" @default.
- W2023997802 isRetracted "false" @default.
- W2023997802 magId "2023997802" @default.
- W2023997802 workType "article" @default.