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- W2024004844 endingPage "374" @default.
- W2024004844 startingPage "356" @default.
- W2024004844 abstract "The primordial germ cells (PGCs) in the colonial urochordate Botryllus schlosseri are sequestered in late embryonic stage. PGC-like populations, located at any blastogenic stage in specific niches, inside modules with curtailed lifespan, survive throughout the life of the colony by repeated weekly migration to newly formed buds. This cyclical migration and the lack of specific markers for PGC-like populations are obstacles to the study on PGCs. For that purpose, we isolated the Botryllus DDX1 (BS-DDX1) and characterized it by normal expression patterns and by specific siRNA knockdown experiments. Expression of BS-DDX1 concurrent with BS-Vasa, γ-H2AX, BS-cadherin and phospho-Smad1/5/8, demarcate PGC cells from soma cells and from more differentiated germ cells lineages, which enabled the detection of additional putative transient niches in zooids. Employing BS-cadherin siRNA knockdown, retinoic acid (RA) administration or β-estradiol administration affirmed the BS-Vasa+BS-DDX1+BS-cadherin+γ-H2AX+phospho-Smad1/5/8+ population as the B. schlosseri PGC-like cells. By striving to understand the PGC-like cells trafficking between transient niches along blastogenic cycles, CM-DiI-stained PGC-like enriched populations from late blastogenic stage D zooids were injected into genetically matched colonial ramets at blastogenic stages A or C and their fates were observed for 9 days. Based on the accumulated data, we conceived a novel network of several transient and short lived ‘germ line niches’ that preserve PGCs homeostasis, protecting these cells from the weekly astogenic senescence processes, thus enabling the survival of the PGCs throughout the organism's life." @default.
- W2024004844 created "2016-06-24" @default.
- W2024004844 creator A5001846283 @default.
- W2024004844 creator A5071498547 @default.
- W2024004844 creator A5090421412 @default.
- W2024004844 creator A5020743993 @default.
- W2024004844 date "2013-12-01" @default.
- W2024004844 modified "2023-10-18" @default.
- W2024004844 title "Germ lineage properties in the urochordate Botryllus schlosseri – From markers to temporal niches" @default.
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