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• W2024083102 abstract "A method for the accurate determination of the fraction of surface-attached DNA duplexes exhibiting a single-fluorophore-labeled nucleobase is introduced. The fluorescence signals obtained from surface plasmon field-enhanced fluorescence spectroscopy along with the optical properties of the sensor architecture determined by surface plasmon resonance were employed for the calculation. A Cy5-labeled nucleotide was incorporated into DNA at a well-defined position via template-directed DNA synthesis performed by a DNA polymerase. The sample-to-sample variations associated with the optical properties of the employed metal films caused a small variation in the strength of the evanescent field. This variation was accounted for by evanescent field integration over the DNA layers. The exponential-type relationship between the fraction of DNA with Cy5-dCTP incorporation at the surface and the mole fraction of the Cy5-dCTP in solution indicates the preferential incorporation of nonlabeled nucleotides by the DNA polymerase." @default.
• W2024083102 created "2016-06-24" @default.
• W2024083102 creator A5031137637 @default.
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• W2024083102 date "2004-07-17" @default.
• W2024083102 modified "2023-10-13" @default.
• W2024083102 title "Concentration of Dye-Labeled Nucleotides Incorporated into DNA Determined by Surface Plasmon Resonance-Surface Plasmon Fluorescence Spectroscopy" @default.
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• W2024083102 doi "https://doi.org/10.1021/ac0495586" @default.
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