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- W2024100034 abstract "The effects of scombroid insulin (10−7 M) and bovine glucagon (10−7 M) on key regulatory enzymes of glycolysis and gluconeogenesis are examined in freshly isolated hepatocytes of the sea raven, Hemitripterus americanus, following a 45 and 180 min incubation. Glucagon increased the Vopt of PEPCK, PK, GPT, and LDH, but decreased the substrate activity ratios of both PK and PFK-1 (the latter only at inhibitory ATP concentrations) at 180 min. Glucagon also decreased Fru-2,6-P2 levels at both 45 and 180 min. Insulin decreased the Vopt for LDH at 180 min and decreased the F6P activity ratio for PFK-1 at both 0.5 mM and 1.5 mM ATP at 45 and 180 min, with larger decreases occurring at the later time period (to 13% of the control value). Insulin also decreased Fru-2,6-P2 concentrations at 180 min and increased alanine uptake. PEPCK and PK were not affected by insulin. These data indicate that glucagon alters the characteristics of a variety of enzymes in the glycolytic/gluconeogenic sequence, while the major impact of insulin is at the level of PFK-1 and alanine uptake." @default.
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- W2024100034 title "Control of key carbohydrate-metabolizing enzymes by insulin and glucagon in freshly isolated hepatocytes of the Marine teleostHemitripterus americanus" @default.
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- W2024100034 doi "https://doi.org/10.1002/jez.1402540109" @default.
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