Matches in SemOpenAlex for { <https://semopenalex.org/work/W20241282> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W20241282 abstract "Expressed sequence tags (ESTs) are partial DNA sequences generated from either the 5 ' or the 3 ' end of cDNA clones. Many large scale cDNA sequencing projects generating thousands of ESTs have been performed. Such EST collections can reflect a substantial proportion of the expressed genes of a species under a given set of conditions. Two potato cDNA libraries derived from pathogen challenged tissue were enriched by virtual subtraction and single pass sequencing of selected clones resulted in 4,795 EST sequences. 4,184 unigenes (3,305 singletons + 879 contigs) were discovered from grouping the ESTs into clusters and contigs. The virtual subtraction enrichment before sequencing of the cDNA libraries was found to be highly effective at reducing EST redundancy and enriching for ESTs of genes expressed at low levels, namely transcription factor genes. As much as 30 fold decreases in the numbers of ESTs representing certain highly expressed genes were observed while some transcription factor ESTs were up to 10 times more abundant than in public EST sets developed from randomly selected clones. In addition, the EST collection was analyzed for percent full length cDNA composition corresponding to genes of different lengths. A potato microarray was constructed from the enriched set of cDNA clones. The B2 gene of potato, a gene believed to be involved in its disease response, was cloned into the gene overexpression vector pRD526 and into the RNAi gene silencing vector pDARTHVECTOR. The constructs were each transformed into Agrobacterium tumefaciens . Future studies conducted using transgenic plants made from these constructs and the microarray will enable the elucidation of the function of B2 through the discovery of interactions between B2 and other disease response genes." @default.
- W20241282 created "2016-06-24" @default.
- W20241282 creator A5025732676 @default.
- W20241282 date "2004-01-01" @default.
- W20241282 modified "2023-09-27" @default.
- W20241282 title "An EST-based genomics project in potato : solanum tuberosum" @default.
- W20241282 hasPublicationYear "2004" @default.
- W20241282 type Work @default.
- W20241282 sameAs 20241282 @default.
- W20241282 citedByCount "0" @default.
- W20241282 crossrefType "dissertation" @default.
- W20241282 hasAuthorship W20241282A5025732676 @default.
- W20241282 hasConcept C104317684 @default.
- W20241282 hasConcept C141231307 @default.
- W20241282 hasConcept C149034497 @default.
- W20241282 hasConcept C150194340 @default.
- W20241282 hasConcept C187882448 @default.
- W20241282 hasConcept C189482833 @default.
- W20241282 hasConcept C2780330800 @default.
- W20241282 hasConcept C54355233 @default.
- W20241282 hasConcept C59582021 @default.
- W20241282 hasConcept C70721500 @default.
- W20241282 hasConcept C86803240 @default.
- W20241282 hasConcept C95371953 @default.
- W20241282 hasConceptScore W20241282C104317684 @default.
- W20241282 hasConceptScore W20241282C141231307 @default.
- W20241282 hasConceptScore W20241282C149034497 @default.
- W20241282 hasConceptScore W20241282C150194340 @default.
- W20241282 hasConceptScore W20241282C187882448 @default.
- W20241282 hasConceptScore W20241282C189482833 @default.
- W20241282 hasConceptScore W20241282C2780330800 @default.
- W20241282 hasConceptScore W20241282C54355233 @default.
- W20241282 hasConceptScore W20241282C59582021 @default.
- W20241282 hasConceptScore W20241282C70721500 @default.
- W20241282 hasConceptScore W20241282C86803240 @default.
- W20241282 hasConceptScore W20241282C95371953 @default.
- W20241282 hasLocation W202412821 @default.
- W20241282 hasOpenAccess W20241282 @default.
- W20241282 hasPrimaryLocation W202412821 @default.
- W20241282 hasRelatedWork W187344178 @default.
- W20241282 hasRelatedWork W1968321933 @default.
- W20241282 hasRelatedWork W1976814946 @default.
- W20241282 hasRelatedWork W1982562255 @default.
- W20241282 hasRelatedWork W1984482057 @default.
- W20241282 hasRelatedWork W1987628567 @default.
- W20241282 hasRelatedWork W1993042573 @default.
- W20241282 hasRelatedWork W2069264113 @default.
- W20241282 hasRelatedWork W2104168496 @default.
- W20241282 hasRelatedWork W2126090536 @default.
- W20241282 hasRelatedWork W2146282656 @default.
- W20241282 hasRelatedWork W2157215359 @default.
- W20241282 hasRelatedWork W2358473194 @default.
- W20241282 hasRelatedWork W2360080710 @default.
- W20241282 hasRelatedWork W2370019515 @default.
- W20241282 hasRelatedWork W2378298844 @default.
- W20241282 hasRelatedWork W2379434698 @default.
- W20241282 hasRelatedWork W2389240430 @default.
- W20241282 hasRelatedWork W2395136821 @default.
- W20241282 hasRelatedWork W994263302 @default.
- W20241282 isParatext "false" @default.
- W20241282 isRetracted "false" @default.
- W20241282 magId "20241282" @default.
- W20241282 workType "dissertation" @default.