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• W2024131142 abstract "Genetic analyses based on chromosomal lac fusions to nitrogen fixation (nif) genes demonstrated that NifA-dependent transcriptional activation of expression of Rhodobacter capsulatus nifH and nifB1 was negatively modulated by HvrA, whereas regulation of rpoN, nifA1, and nifA2 was independent of HvrA. Expression of hvrA itself was not influenced by a mutation in ntrC, which is absolutely essential for N(2) fixation. Furthermore, HvrA accumulated to comparable levels in the presence and absence of ammonium, suggesting that the amount of HvrA in the cells does not differ under nitrogenase-repressing or -derepressing conditions. In addition, competitive gel retardation studies with HvrA-His(6) purified from R. capsulatus were carried out, demonstrating preferential binding of HvrA to the nifH promoter region." @default.
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• W2024131142 date "2002-11-01" @default.
• W2024131142 modified "2023-09-26" @default.
• W2024131142 title "The H-NS-like protein HvrA modulates expression of nitrogen fixation genes in the phototrophic purple bacterium<i>Rhodobacter capsulatus</i>by binding to selected<i>nif</i>promoters" @default.
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• W2024131142 doi "https://doi.org/10.1111/j.1574-6968.2002.tb11429.x" @default.
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