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- W2024138730 abstract "A simple method for isolating the nuclei from Basidiobolus ranarum was established. To improve the yield and purity of nuclei, we investigated maceration methods, buffer composition, and centrifugation conditions to establish an optimal procedure. Basidiobolus ranarum cultured for 5 days was enzymatically macerated and then homogenized and filtrated through stainless steel sieves. The crude cell homogenate was loaded on a layer of buffer containing 50% glycerol and centrifuged at 1500 g. The resultant pellet contained pure nuclei." @default.
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- W2024138730 date "2009-11-01" @default.
- W2024138730 modified "2023-09-26" @default.
- W2024138730 title "A simple method for isolation of nuclei from Basidiobolus ranarum (Zygomycota)" @default.
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- W2024138730 doi "https://doi.org/10.1007/s10267-009-0501-5" @default.
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