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• W2024143783 abstract "We probe DNA hybridization kinetics by measuring the lifetime distribution of single 16-bp duplexes under thermal dissociation. Our unique approach, based on two DNA-coated microspheres in an extended optical tweezer, allows the study of single duplex DNA molecules under negligible molecular tension. In contrast to earlier experiments, we find a stretched exponential lifetime distribution, which is likely due to dissociation proceeding via a number of competing pathways between highly force-sensitive intermediate states. Similar measurements of microspheres linked by multiple DNA bridges find they have unexpected short bound lifetimes, also consistent with force sensitivity." @default.
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• W2024143783 date "2008-02-01" @default.
• W2024143783 modified "2023-10-12" @default.
• W2024143783 title "Long-Time Stretched Exponential Kinetics in Single DNA Duplex Dissociation" @default.
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• W2024143783 doi "https://doi.org/10.1529/biophysj.107.108449" @default.
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