FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024149997> ?p ?o ?g. }

• W2024149997 endingPage "770" @default.
• W2024149997 startingPage "764" @default.
• W2024149997 abstract "ABSTRACT Organic solvent extracts of whole cells of the gram-positive bacterium Rhodococcus erythropolis contain a channel-forming protein. It was identified by lipid bilayer experiments and purified to homogeneity by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). The pure protein had a rather low molecular mass of about 8.4 kDa, as judged by SDS-PAGE. SDS-resistant oligomers with a molecular mass of 67 kDa were also observed, suggesting that the channel is formed by a protein oligomer. The monomer was subjected to partial protein sequencing, and 45 amino acids were resolved. According to the partial sequence, the sequence has no significant homology to known protein sequences. To check whether the channel was indeed localized in the cell wall, the cell wall fraction was separated from the cytoplasmic membrane by sucrose step gradient centrifugation. The highest channel-forming activity was found in the cell wall fraction. The purified protein formed large ion-permeable channels in lipid bilayer membranes with a single-channel conductance of 6.0 nS in 1 M KCl. Zero-current membrane potential measurements with different salts suggested that the channel of R. erythropolis was highly cation selective because of negative charges localized at the channel mouth. The correction of single-channel conductance data for negatively charged point charges and the Renkin correction factor suggested that the diameter of the cell wall channel is about 2.0 nm. The channel-forming properties of the cell wall channel of R. erythropolis were compared with those of other members of the mycolata. These channels have common features because they form large, water-filled channels that contain net point charges." @default.
• W2024149997 created "2016-06-24" @default.
• W2024149997 creator A5052342777 @default.
• W2024149997 creator A5068203602 @default.
• W2024149997 creator A5069862319 @default.
• W2024149997 date "2000-02-01" @default.
• W2024149997 modified "2023-10-08" @default.
• W2024149997 title "Biochemical Identification and Biophysical Characterization of a Channel-Forming Protein from <i>Rhodococcus erythropolis</i>" @default.
• W2024149997 cites W1007654216 @default.
• W2024149997 cites W1526283615 @default.
• W2024149997 cites W1528773391 @default.
• W2024149997 cites W1548110598 @default.
• W2024149997 cites W1588625450 @default.
• W2024149997 cites W1908882478 @default.
• W2024149997 cites W1973369594 @default.
• W2024149997 cites W1975085160 @default.
• W2024149997 cites W1979672173 @default.
• W2024149997 cites W1983492677 @default.
• W2024149997 cites W1989709416 @default.
• W2024149997 cites W2000370325 @default.
• W2024149997 cites W2005521991 @default.
• W2024149997 cites W2006986579 @default.
• W2024149997 cites W2012933005 @default.
• W2024149997 cites W2021055207 @default.
• W2024149997 cites W2021793865 @default.
• W2024149997 cites W2027100088 @default.
• W2024149997 cites W2038031211 @default.
• W2024149997 cites W2038536885 @default.
• W2024149997 cites W2040504954 @default.
• W2024149997 cites W2044428175 @default.
• W2024149997 cites W2044853699 @default.
• W2024149997 cites W2061768343 @default.
• W2024149997 cites W2066815397 @default.
• W2024149997 cites W2075727323 @default.
• W2024149997 cites W2076200938 @default.
• W2024149997 cites W2103556163 @default.
• W2024149997 cites W2132130047 @default.
• W2024149997 cites W2134139057 @default.
• W2024149997 cites W2140981202 @default.
• W2024149997 cites W2143988825 @default.
• W2024149997 cites W2151070439 @default.
• W2024149997 cites W2151643412 @default.
• W2024149997 cites W2159370857 @default.
• W2024149997 cites W2161681336 @default.
• W2024149997 cites W4239764402 @default.
• W2024149997 doi "https://doi.org/10.1128/jb.182.3.764-770.2000" @default.
• W2024149997 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/94341" @default.
• W2024149997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10633112" @default.
• W2024149997 hasPublicationYear "2000" @default.
• W2024149997 type Work @default.
• W2024149997 sameAs 2024149997 @default.
• W2024149997 citedByCount "41" @default.
• W2024149997 countsByYear W20241499972012 @default.
• W2024149997 countsByYear W20241499972013 @default.
• W2024149997 countsByYear W20241499972014 @default.
• W2024149997 countsByYear W20241499972015 @default.
• W2024149997 countsByYear W20241499972017 @default.
• W2024149997 countsByYear W20241499972018 @default.
• W2024149997 countsByYear W20241499972019 @default.
• W2024149997 countsByYear W20241499972020 @default.
• W2024149997 countsByYear W20241499972022 @default.
• W2024149997 countsByYear W20241499972023 @default.
• W2024149997 crossrefType "journal-article" @default.
• W2024149997 hasAuthorship W2024149997A5052342777 @default.
• W2024149997 hasAuthorship W2024149997A5068203602 @default.
• W2024149997 hasAuthorship W2024149997A5069862319 @default.
• W2024149997 hasBestOaLocation W20241499972 @default.
• W2024149997 hasConcept C104317684 @default.
• W2024149997 hasConcept C116084860 @default.
• W2024149997 hasConcept C125235067 @default.
• W2024149997 hasConcept C12554922 @default.
• W2024149997 hasConcept C132946820 @default.
• W2024149997 hasConcept C178790620 @default.
• W2024149997 hasConcept C181199279 @default.
• W2024149997 hasConcept C185592680 @default.
• W2024149997 hasConcept C190062978 @default.
• W2024149997 hasConcept C192157962 @default.
• W2024149997 hasConcept C2780642029 @default.
• W2024149997 hasConcept C39944091 @default.
• W2024149997 hasConcept C41625074 @default.
• W2024149997 hasConcept C43617362 @default.
• W2024149997 hasConcept C46371251 @default.
• W2024149997 hasConcept C547475151 @default.
• W2024149997 hasConcept C55493867 @default.
• W2024149997 hasConcept C58121356 @default.
• W2024149997 hasConcept C86803240 @default.
• W2024149997 hasConcept C96294017 @default.
• W2024149997 hasConceptScore W2024149997C104317684 @default.
• W2024149997 hasConceptScore W2024149997C116084860 @default.
• W2024149997 hasConceptScore W2024149997C125235067 @default.
• W2024149997 hasConceptScore W2024149997C12554922 @default.
• W2024149997 hasConceptScore W2024149997C132946820 @default.
• W2024149997 hasConceptScore W2024149997C178790620 @default.
• W2024149997 hasConceptScore W2024149997C181199279 @default.
• W2024149997 hasConceptScore W2024149997C185592680 @default.
• W2024149997 hasConceptScore W2024149997C190062978 @default.
• W2024149997 hasConceptScore W2024149997C192157962 @default.
• W2024149997 hasConceptScore W2024149997C2780642029 @default.

• next