FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024150300> ?p ?o ?g. }

• W2024150300 endingPage "1953" @default.
• W2024150300 startingPage "1943" @default.
• W2024150300 abstract "The cofactor-binding domains (residues 153−295) of the alcohol dehydrogenases from the thermophile Thermoanaerobacter brockii (TbADH), the mesophilic bacterium Clostridium beijerinckii (CbADH), and the protozoan parasite Entamoeba histolytica (EhADH1) have been exchanged. Three chimeras have been constructed. In the first chimera, the cofactor-binding domain of thermophilic TbADH was replaced with the cofactor-binding domain of its mesophilic counterpart CbADH [chimera Χ21(TCT)]. This domain exchange significantly destabilized the parent thermophilic enzyme (ΔT1/2 = −18 °C). The reverse exchange in CbADH [chimera Χ22(CTC)], however, had little effect on the thermal stability of the parent mesophilic protein. Furthermore, substituting the cofactor-binding domain of TbADH with the homologous domain of EhADH1 [chimera Χ23(TET)] substantially reduced the thermal stability of the thermophilic ADH (ΔT1/2 = −51 °C) and impeded the oligomerization of the enzyme. All three chimeric proteins and one of their site-directed mutants were crystallized, and their three-dimensional (3D) structures were determined. Comparison of the 3D structures of the chimeras and the chimeric mutant with the structures of their parent ADHs showed no significant changes to their Cα chains, suggesting that the difference in the thermal stability of the three parent ADHs and their chimeric mutants could be due to a limited number of substitutions located at strategic positions, mainly at the oligomerization interfaces. Indeed, stabilization of the chimeras was achieved, to a significant extent, either by introduction of a proline residue at a strategic position in the major horse liver ADH-type dimerization interface (ΔT1/2 = 35 °C) or by introduction of intersubunit electrostatic interactions (ΔT1/2 = 6 °C)." @default.
• W2024150300 created "2016-06-24" @default.
• W2024150300 creator A5001923252 @default.
• W2024150300 creator A5002223698 @default.
• W2024150300 creator A5017797758 @default.
• W2024150300 creator A5022394866 @default.
• W2024150300 creator A5027545846 @default.
• W2024150300 creator A5058203625 @default.
• W2024150300 creator A5071495846 @default.
• W2024150300 date "2010-02-09" @default.
• W2024150300 modified "2023-09-26" @default.
• W2024150300 title "Biochemical and Structural Properties of Chimeras Constructed by Exchange of Cofactor-Binding Domains in Alcohol Dehydrogenases from Thermophilic and Mesophilic Microorganisms" @default.
• W2024150300 cites W1506664007 @default.
• W2024150300 cites W1520730837 @default.
• W2024150300 cites W1539796472 @default.
• W2024150300 cites W1549709155 @default.
• W2024150300 cites W1985842673 @default.
• W2024150300 cites W1986191025 @default.
• W2024150300 cites W1986550844 @default.
• W2024150300 cites W1996607909 @default.
• W2024150300 cites W1999147504 @default.
• W2024150300 cites W2001210310 @default.
• W2024150300 cites W2002028486 @default.
• W2024150300 cites W2005067538 @default.
• W2024150300 cites W2005539780 @default.
• W2024150300 cites W2008450196 @default.
• W2024150300 cites W2010330695 @default.
• W2024150300 cites W2015279243 @default.
• W2024150300 cites W2022081324 @default.
• W2024150300 cites W2023751078 @default.
• W2024150300 cites W2029568869 @default.
• W2024150300 cites W2031064526 @default.
• W2024150300 cites W2031238062 @default.
• W2024150300 cites W2031778321 @default.
• W2024150300 cites W2037695417 @default.
• W2024150300 cites W2040758408 @default.
• W2024150300 cites W2048411275 @default.
• W2024150300 cites W2049576685 @default.
• W2024150300 cites W2055010078 @default.
• W2024150300 cites W2058715178 @default.
• W2024150300 cites W2076842629 @default.
• W2024150300 cites W2092784580 @default.
• W2024150300 cites W2094823743 @default.
• W2024150300 cites W2097493124 @default.
• W2024150300 cites W2100837269 @default.
• W2024150300 cites W2105590412 @default.
• W2024150300 cites W2108305553 @default.
• W2024150300 cites W2108897255 @default.
• W2024150300 cites W2122339645 @default.
• W2024150300 cites W2132717858 @default.
• W2024150300 cites W2134393386 @default.
• W2024150300 cites W2153290910 @default.
• W2024150300 cites W2163627794 @default.
• W2024150300 cites W2164548274 @default.
• W2024150300 cites W2169346156 @default.
• W2024150300 cites W4293247451 @default.
• W2024150300 cites W9996748 @default.
• W2024150300 doi "https://doi.org/10.1021/bi901730x" @default.
• W2024150300 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20102159" @default.
• W2024150300 hasPublicationYear "2010" @default.
• W2024150300 type Work @default.
• W2024150300 sameAs 2024150300 @default.
• W2024150300 citedByCount "9" @default.
• W2024150300 countsByYear W20241503002012 @default.
• W2024150300 countsByYear W20241503002013 @default.
• W2024150300 countsByYear W20241503002015 @default.
• W2024150300 countsByYear W20241503002016 @default.
• W2024150300 countsByYear W20241503002017 @default.
• W2024150300 countsByYear W20241503002018 @default.
• W2024150300 countsByYear W20241503002021 @default.
• W2024150300 crossrefType "journal-article" @default.
• W2024150300 hasAuthorship W2024150300A5001923252 @default.
• W2024150300 hasAuthorship W2024150300A5002223698 @default.
• W2024150300 hasAuthorship W2024150300A5017797758 @default.
• W2024150300 hasAuthorship W2024150300A5022394866 @default.
• W2024150300 hasAuthorship W2024150300A5027545846 @default.
• W2024150300 hasAuthorship W2024150300A5058203625 @default.
• W2024150300 hasAuthorship W2024150300A5071495846 @default.
• W2024150300 hasConcept C104317684 @default.
• W2024150300 hasConcept C143065580 @default.
• W2024150300 hasConcept C181199279 @default.
• W2024150300 hasConcept C185592680 @default.
• W2024150300 hasConcept C197957613 @default.
• W2024150300 hasConcept C2776943354 @default.
• W2024150300 hasConcept C2777344606 @default.
• W2024150300 hasConcept C2781216036 @default.
• W2024150300 hasConcept C27911776 @default.
• W2024150300 hasConcept C30278631 @default.
• W2024150300 hasConcept C523546767 @default.
• W2024150300 hasConcept C54355233 @default.
• W2024150300 hasConcept C550995028 @default.
• W2024150300 hasConcept C55493867 @default.
• W2024150300 hasConcept C63223902 @default.
• W2024150300 hasConcept C86803240 @default.
• W2024150300 hasConceptScore W2024150300C104317684 @default.
• W2024150300 hasConceptScore W2024150300C143065580 @default.
• W2024150300 hasConceptScore W2024150300C181199279 @default.
• W2024150300 hasConceptScore W2024150300C185592680 @default.

• next