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- W2024156843 endingPage "288" @default.
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- W2024156843 abstract "Photosystem II is a multimeric protein complex of the thylakoid membrane in chloroplasts. Approximately half of the at least 26 different integral membrane protein subunits have molecular masses lower than 10 kDa. After one-dimensional (1D) or two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE) separation, followed by enzymatic digestion of detected proteins, hardly any of these low-molecular-weight (LMW) subunits are detectable. Therefore, we developed a method for the analysis of highly hydrophobic LMW proteins. Intact proteins are extracted from acrylamide gels using a mixture of formic acid and organic solvent, precipitated with acetone, and analyzed by “top-down” mass spectrometry (MS). After offline nanoESI (electrospray ionization) MS, all LMW one-helix proteins from photosystem II were detected. In the four detected photosystem II supercomplexes of Nicotiana tabacum wild-type plants, 11 different one-helix proteins were identified as PsbE, -F, -H, -I, -K, -L, -M, -Tc, -W, and two isoforms of PsbX. The proteins PsbJ, -Y1, and -Y2 were localized in the buffer front after blue native (BN) PAGE, indicating their release during solubilization. Assembled PsbW is detected exclusively in supercomplexes, whereas it is absent in photosystem II core complexes, corroborating the protein’s function for assembly of the light-harvesting complexes. This approach will substantiate gel-blot immunoanalysis for localization and identification of LMW protein subunits in any membrane protein complex." @default.
- W2024156843 created "2016-06-24" @default.
- W2024156843 creator A5016799532 @default.
- W2024156843 creator A5046447942 @default.
- W2024156843 creator A5055733001 @default.
- W2024156843 creator A5074737892 @default.
- W2024156843 date "2008-12-01" @default.
- W2024156843 modified "2023-10-17" @default.
- W2024156843 title "Localization of 13 one-helix integral membrane proteins in photosystem II subcomplexes" @default.
- W2024156843 cites W1521146215 @default.
- W2024156843 cites W1558365920 @default.
- W2024156843 cites W1574529934 @default.
- W2024156843 cites W1576807287 @default.
- W2024156843 cites W1588004899 @default.
- W2024156843 cites W1601113496 @default.
- W2024156843 cites W1736022414 @default.
- W2024156843 cites W1961714136 @default.
- W2024156843 cites W1963507667 @default.
- W2024156843 cites W1963530308 @default.
- W2024156843 cites W1964340809 @default.
- W2024156843 cites W1966113050 @default.
- W2024156843 cites W1967977024 @default.
- W2024156843 cites W1974719543 @default.
- W2024156843 cites W1975304761 @default.
- W2024156843 cites W1978609421 @default.
- W2024156843 cites W1991358290 @default.
- W2024156843 cites W1991877682 @default.
- W2024156843 cites W1991963818 @default.
- W2024156843 cites W1992714409 @default.
- W2024156843 cites W1993204359 @default.
- W2024156843 cites W1996316908 @default.
- W2024156843 cites W1998098749 @default.
- W2024156843 cites W1998883018 @default.
- W2024156843 cites W1999128746 @default.
- W2024156843 cites W1999498185 @default.
- W2024156843 cites W2003375273 @default.
- W2024156843 cites W2005431453 @default.
- W2024156843 cites W2006986579 @default.
- W2024156843 cites W2008455501 @default.
- W2024156843 cites W2008756585 @default.
- W2024156843 cites W2009516943 @default.
- W2024156843 cites W2012076237 @default.
- W2024156843 cites W2013417148 @default.
- W2024156843 cites W2013785625 @default.
- W2024156843 cites W2014405882 @default.
- W2024156843 cites W2015522317 @default.
- W2024156843 cites W2021071169 @default.
- W2024156843 cites W2024556021 @default.
- W2024156843 cites W2026465178 @default.
- W2024156843 cites W2027492037 @default.
- W2024156843 cites W2028383530 @default.
- W2024156843 cites W2030595802 @default.
- W2024156843 cites W2039795593 @default.
- W2024156843 cites W2043617852 @default.
- W2024156843 cites W2045622515 @default.
- W2024156843 cites W2049101506 @default.
- W2024156843 cites W2057863409 @default.
- W2024156843 cites W2058128046 @default.
- W2024156843 cites W2061765768 @default.
- W2024156843 cites W2070292711 @default.
- W2024156843 cites W2073317899 @default.
- W2024156843 cites W2077215543 @default.
- W2024156843 cites W2079049976 @default.
- W2024156843 cites W2080750661 @default.
- W2024156843 cites W2083907491 @default.
- W2024156843 cites W2085101416 @default.
- W2024156843 cites W2087968430 @default.
- W2024156843 cites W2098476715 @default.
- W2024156843 cites W2100321396 @default.
- W2024156843 cites W2103770181 @default.
- W2024156843 cites W2107919301 @default.
- W2024156843 cites W2111227548 @default.
- W2024156843 cites W2117600679 @default.
- W2024156843 cites W2119376397 @default.
- W2024156843 cites W2123061116 @default.
- W2024156843 cites W2126423510 @default.
- W2024156843 cites W2128671236 @default.
- W2024156843 cites W2129095580 @default.
- W2024156843 cites W2142331686 @default.
- W2024156843 cites W2147844147 @default.
- W2024156843 cites W2149206092 @default.
- W2024156843 cites W2151756922 @default.
- W2024156843 cites W2152940258 @default.
- W2024156843 cites W2156986654 @default.
- W2024156843 cites W2161574105 @default.
- W2024156843 cites W2167617806 @default.
- W2024156843 cites W4245907455 @default.
- W2024156843 cites W8906737 @default.
- W2024156843 doi "https://doi.org/10.1016/j.ab.2008.08.038" @default.
- W2024156843 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18804444" @default.
- W2024156843 hasPublicationYear "2008" @default.
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