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- W2024162582 abstract "Gas chromatography revealed that exposure of extracts of the denitrifiers ‘Achromobacter cycloclastes’, Paracoccus denitrificans, Pseudomonas aeruginosa and Pseudomonas perfectomarina to Triton X-100 inhibited reduction of NO to N2O, and thus concomitantly inhibited reduction of NO2− to N2O. After exposure of extracts to Triton X-100, the ratio of H+ consumed to NO2− added decreased from approx. 2.0 (for untreated extracts) to approx. 1.5, which indicated that NO2− was reduced to NO by the treated extracts. Addition of a CHAPS-soluble extracts (devoid of nitrite reductase activity but rich in nitric-oxide reductase activity) to the Triton X-100-treated extract of P. denitrificans restored capacity for reduction of NO2− on to N2O. Exposure to either the NO that accumulated from reduction of NO2− or to enthetic NO transiently inhibited rates of NO2− reduction in Triton X-100-treated extracts. Use of an Oxides of Nitrogen analyzer indicated that only 5–33% of NO2− reduced by untreated extracts appeared in the stripping gas as NO, whereas 80–95% of NO2− reduced by Triton X-100-treated extracts was recovered as NO." @default.
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- W2024162582 date "1987-02-01" @default.
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- W2024162582 title "Detergent inhibition of nitric-oxide reductase activity" @default.
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- W2024162582 doi "https://doi.org/10.1016/0167-4838(87)90074-4" @default.
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