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• W2024171602 abstract "Cytochrome P450 2A6 (CYP2A6) is a polymorphic enzyme responsible for the oxidation of certain precarcinogens and drugs and is the major nicotine C-oxidase. The role of CYP2A6 for nicotine elimination was emphasised recently by the finding that smokers carrying defective CYP2A6 alleles consumed fewer cigarettes [Pianezza et al. (1998) Nature 393, 750]. The method used for CYP2A6 genotyping has, however, been found to give erroneous results with respect to the coumarin hydroxylase phenotype, a probe reaction for the CYP2A6 enzyme. The present study describes an allele-specific PCR genotyping method that identifies the major defective CYP2A6 allele and accurately predicts the phenotype. An allele frequency of 1-3% was observed in Finnish, Spanish, and Swedish populations, much lower than described previously." @default.
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• W2024171602 date "1998-11-06" @default.
• W2024171602 modified "2023-10-18" @default.
• W2024171602 title "Genotyping of human cytochrome P450 2A6 (CYP2A6), a nicotine <i>C</i> -oxidase" @default.
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• W2024171602 doi "https://doi.org/10.1016/s0014-5793(98)01297-6" @default.
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