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- W2024182002 abstract "A solid phase radioimmunpassay for the measurement of aldosterone in plasma is described. The antiserum was produced by immunizing rabbits with 3-carboxymethyloxime of aldosterone-18-21-diacetate coupled to bovine serum albumin, This antiserum was covalently linked to an iminocellulose according to the procedure of Wide and used in the assay at a 11050 final dilution. It contained antibodies with association-constant of 1.1 × 1010M−1 and was fairly specific since with the exception of aldosterone acetates, none of the tested steroids cross-reacted more than 0.05 per cent. Aldosterone was extracted with dichloromethane, purified by paper chromatography, then submitted to the assay. The intra-assay reproducibility varied between 4 and 13 % and the inter-assay reproducibility between 13 and 21 %. The least detectable amount was 5 pg per tube. This method is very simple and, with the exception of the Chromatographie step, can be completed in half a working day." @default.
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- W2024182002 date "1974-06-01" @default.
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- W2024182002 title "Solid phase radioimmunoassay of plasma aldosterone" @default.
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- W2024182002 doi "https://doi.org/10.1016/0039-128x(74)90055-5" @default.
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