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- W2024184610 abstract "Seminiferous tubules of rat testes were exposed to distilled water to lyse the interstitial cells. After homogenization in isotonic phosphate saline, intact viable germ cells were recovered. Straining through a layer of Sephadex-G 25 or glasswool removed flagellate cells and Sertoli cells. The germ cells were separated by velocity sedimentation in a sucrose gradient at 1 × g into spermatocyte and spermatid enriched fractions. The spermatids were further resolved into round and elongating spermatids by centrifugation in an isopycnic Ficoll gradient. The absence of Leydig and Sertoli cells from the germ cell suspensions was confirmed by cytological and histochemical criteria. Incubation of the cell fractions with progesterone showed that the ability of the germ cells to convert progesterone to testosterone resided in the spermatocyte enriched fraction. This fraction also produced testosterone on incubation without added steroid precursor and with slight stimulation by added LH. These findings suggest that germ cells at the primary spermatocyte stage have the capability to produce androgen not only from external progesterone, but also from endogenous steroid precursors. The androgen content of rete testis fluid may be augmented to a limited, but significant, extent by the activity of intratubular cells." @default.
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- W2024184610 date "1979-09-01" @default.
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- W2024184610 title "Androgen Synthesis in Absence of Leydig and Sertoli Cells in a Germ Cell Fraction from Rat Seminiferous Tubules1" @default.
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- W2024184610 doi "https://doi.org/10.1095/biolreprod21.2.293" @default.
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